Abstract：
The Siddha system of medicine (SSM) is the oldest medical science practised in the ancient period of the southern part of India and Sri Lanka. Many formulations were described for wound healing in the SSM, with specific diagnostic differentiation in the Siddha literature. Most preparations for wound healing were available in the form of oil-based formulations, especially for external usage. Mathan tailam (MT) and Mahamegarajanga tailam (MMRT) have been used by Siddha physicians and traditional practitioners to treat wounds. Mathan tailam is a popular regimen for skin lacerations, burns, skin infections, diabetic wounds, and dermatitis. Mahamegarajanga tailam has long been used by traditional vaidyars to treat cuts and burns. Both MT and MMRT are clinically well-appreciated drugs for wound healing and need to be studied for their mechanisms of action for scientific documentation. In an in vivo study on albino rats -excisional wound model, the histopathological changes, histo-immune response, biomarker analysis, and mRNA expression were studied and analysed. Wounds treated with MT and MMRT healed faster (p < 0.05) than the untreated group (CNT). Histological investigation showed rapid re-epithelialization, dense collagen deposition, increased enzymatic antioxidant activities and decreased lipid peroxidation in the MT and MMRT groups. mRNA expression reveals MT and MMRT-treated tissues able to induce convergent cell motility in wound space. Our study for the first time provides strong in vivo experimental evidence that Mathan tailam and Mahamegarajanga tailam play a crucial role in promoting skin tissue wound healing through IL-6/VEGF/TNF-α mediated mechanisms. Traditional practices continue to teach us valuable lessons, as seen by their continuous use in their locality for years.
摘要：
Siddha医学系统（SSM）是印度和斯里兰卡南部古代时期最古老的医学科学。在SSM中描述了许多用于伤口愈合的制剂，在Siddha文献中具有特定的诊断分化。大多数用于伤口愈合的制剂以油基制剂的形式获得，特别是外部使用。Siddha医师和传统从业者已使用Matantailam（MT）和Mahamegarajangatailam（MMRT）来治疗伤口。Mathantailam是一种流行的皮肤撕裂疗法，烧伤,皮肤感染,糖尿病伤口,和皮炎。Mahamegarajangatalam长期以来一直被传统的vaidyars用于治疗割伤和烧伤。MT和MMRT都是临床上公认的伤口愈合药物，需要研究其作用机制以提供科学文献。在白化病大鼠切除伤口模型的体内研究中，组织病理学变化,组织免疫反应，生物标志物分析,和mRNA表达进行了研究和分析。用MT和MMRT处理的伤口比未处理组(CNT)愈合更快(p<0.05)。组织学调查显示快速上皮重新形成，致密的胶原蛋白沉积，MT和MMRT组的酶促抗氧化活性增加，脂质过氧化降低。mRNA表达揭示MT和MMRT处理的组织能够诱导伤口空间中的会聚细胞运动。我们的研究首次提供了强有力的体内实验证据，表明Mathantailam和Mahamegarajangatailam通过IL-6/VEGF/TNF-α介导的机制在促进皮肤组织伤口愈合中起着至关重要的作用。传统习俗不断给我们宝贵的教训,从它们在当地多年的持续使用中可以看出。
