PDF(Pubmed)
Abstract：
Climate change presents multiple challenges to rural communities. Here, we investigated the toxicological potential of the six types of particulate matter most common to rural Arkansas: soil, road, and agricultural dusts, pollen, traffic exhaust, and particles from biomass burning in human small airway epithelial cells (SAECs). Biomass burning and agricultural dust demonstrated the most potent toxicological responses, exhibited as significant (p < 0.05) up-regulation of HMOX1 (oxidative stress) and TNFα (inflammatory response) genes as well as epigenetic alterations (altered expression of DNA methyltransferases DNMT1, DNMT3A, and DNMT3B, enzymatic activity, and DNA methylation of alpha satellite elements) that were evident at both 24 h and 72 h of exposure. We further demonstrate evidence of aridification in the state of Arkansas and the presence of winds capable of transporting agricultural dust- and biomass burning-associated particles far beyond their origination. Partnerships in the form of citizen science projects may provide important solutions to prevent and mitigate the negative effects of the rapidly evolving climate and improve the well-being of rural communities. Furthermore, the identification of the most toxic types of particulate matter could inform local policies related to agriculture, biomass burning, and dust control.
摘要：
气候变化给农村社区带来了多重挑战。这里,我们调查了阿肯色州农村最常见的六种颗粒物的毒理学潜力：土壤，道路,和农业灰尘，花粉,交通废气,和来自人小气道上皮细胞（SAEC）中生物质燃烧的颗粒。生物质燃烧和农业粉尘表现出最有效的毒理学反应，表现为HMOX1（氧化应激）和TNFα（炎症反应）基因的显着上调（p<0.05）以及表观遗传改变（DNA甲基转移酶DNMT1，DNMT3A的表达改变，和DNMT3B，酶活性，和α卫星元件的DNA甲基化）在暴露的24小时和72小时都很明显。我们进一步证明了阿肯色州干旱化的证据，并且存在能够将与农业粉尘和生物质燃烧相关的颗粒远远超出其起源的风。公民科学项目形式的伙伴关系可以提供重要的解决方案，以预防和减轻迅速变化的气候的负面影响，并改善农村社区的福祉。此外，确定毒性最强的颗粒物类型可以为当地农业相关政策提供信息，生物质燃烧，和粉尘控制。
