Abstract：
The autophagy-lysosomal pathway enables the controlled degradation of cellular contents. Nucleophagy is the selective autophagic recycling of nuclear components upon delivery to the lysosome. Although methods to monitor and quantify autophagy as well as selective types of autophagy have been developed and implemented in cells and in vivo, methods monitoring nucleophagy remain scarce. Here, we describe a procedure to monitor the autophagic engagement of an endogenous nuclear envelope component, i.e., ANC-1, the nematode homologue of the mammalian Nesprins in vivo, utilizing super-resolution microscopy.
摘要：
自噬-溶酶体途径能够控制细胞内容物的降解。核噬是核组分在递送至溶酶体时的选择性自噬再循环。尽管在细胞和体内已经开发并实施了监测和定量自噬以及选择性自噬类型的方法，监测核吞噬的方法仍然很少。这里,我们描述了一个过程来监测内源性核膜成分的自噬参与，即，ANC-1，哺乳动物Nesprins体内的线虫同源物，利用超分辨率显微镜。
