PDF(Pubmed)
Abstract：
UNASSIGNED: Alzheimer\'s disease (AD) is a neurodegenerative condition characterized by gradual cognitive impairment, including loss of synapses and nerve cells involved in learning, memory, and habit formation processes. Bone Marrow Mesenchymal Stem Cells (BM-MSCs) are multipotent cells. Because of their self-renewable, differentiation, and immunomodulatory capabilities, they are commonly used to treat many disorders. Hence, the current study intends to examine the effect of BM-MSCs transplantation on Aluminum chloride (AlCl3)-induced cognitive problems, an experimental model resembling AD\'s hallmarks in rats.
UNASSIGNED: The study was conducted in 2022 at The Biomedical Laboratory Faculty of Medicine, Andalas University, Indonesia. Adult male Wistar rats (three groups: negative control; no intervention+treatment with PBS; positive control: AlCl3+treatment with aqua dest; AlCl3+BM-MSCs: AlCl3+treatment with BM-MSCs, n=5 each) were treated daily with AlCl3 orally for five days. Stem cells were intraperitoneally injected into rats at a dose of 1x106 cells/rat. The same quantity of phosphate-buffered saline was given to the control group. One month after stem cell injection, the rat brain tissue was removed and placed in the film bottles that had been created. The expression of neural progenitor cell markers, including nestin and sex-determining Y-box 2 (SOX-2), was analyzed using real-time polymerase chain reaction (RT-PCR). Rats\' cognitive and functional memory were examined using Y-maze. Data were analyzed using SPSS software (version 26.0) with a one-way analysis of variance (ANOVA) test.
UNASSIGNED: The gene expression of nestin (29.74±0.42), SOX-2 (31.44±0.67), and percent alternation of Y-maze (67.04±2.28) increased in the AlCl3+BM-MSCs group compared to that in the positive control group. RT-PCR analysis indicated that nestin (P<0.001) and SOX-2 (P<0.001) were significantly enhanced in the AlCl3+BM-MSCs group compared to the positive control group. This group also indicated an increased percent alternation of Y-maze (P<0.001) in the AlCl3+BM-MSCs group compared to the positive control group.
UNASSIGNED: Due to its potential effects on cell therapy, BM-MSCs were found effective in a rat model of AD on the impairment of the rats\' behavior and increased expression of neural progenitor cell markers.
UNASSIGNED: The study was conducted in 2022 at The Biomedical Laboratory Faculty of Medicine, Andalas University, Indonesia. Adult male Wistar rats (three groups: negative control; no intervention+treatment with PBS; positive control: AlCl3+treatment with aqua dest; AlCl3+BM-MSCs: AlCl3+treatment with BM-MSCs, n=5 each) were treated daily with AlCl3 orally for five days. Stem cells were intraperitoneally injected into rats at a dose of 1x106 cells/rat. The same quantity of phosphate-buffered saline was given to the control group. One month after stem cell injection, the rat brain tissue was removed and placed in the film bottles that had been created. The expression of neural progenitor cell markers, including nestin and sex-determining Y-box 2 (SOX-2), was analyzed using real-time polymerase chain reaction (RT-PCR). Rats\' cognitive and functional memory were examined using Y-maze. Data were analyzed using SPSS software (version 26.0) with a one-way analysis of variance (ANOVA) test.
UNASSIGNED: The gene expression of nestin (29.74±0.42), SOX-2 (31.44±0.67), and percent alternation of Y-maze (67.04±2.28) increased in the AlCl3+BM-MSCs group compared to that in the positive control group. RT-PCR analysis indicated that nestin (P<0.001) and SOX-2 (P<0.001) were significantly enhanced in the AlCl3+BM-MSCs group compared to the positive control group. This group also indicated an increased percent alternation of Y-maze (P<0.001) in the AlCl3+BM-MSCs group compared to the positive control group.
UNASSIGNED: Due to its potential effects on cell therapy, BM-MSCs were found effective in a rat model of AD on the impairment of the rats\' behavior and increased expression of neural progenitor cell markers.
摘要：
■阿尔茨海默病（AD）是一种神经退行性疾病，其特征是逐渐的认知障碍，包括学习中涉及的突触和神经细胞的丢失，记忆,和习惯形成过程。骨髓间充质干细胞(BM-MSC)是多能细胞。因为它们的自我再生，分化,和免疫调节能力，它们通常用于治疗许多疾病。因此，本研究旨在研究BM-MSCs移植对氯化铝(AlCl3)诱导的认知问题的影响,一种类似于大鼠AD标志的实验模型。
■这项研究于2022年在生物医学实验室医学院进行，安达拉斯大学，印度尼西亚。成年雄性Wistar大鼠（三组：阴性对照；无干预+PBS处理；阳性对照：AlCl3+水处理；AlCl3+BM-MSCs：AlCl3+BM-MSCs处理，每个n=5)每天口服AlCl3治疗五天。将干细胞以1×106个细胞/大鼠的剂量腹膜内注射到大鼠中。对照组给予相同量的磷酸盐缓冲盐水。干细胞注射后一个月,取出大鼠脑组织，并将其放入已制作的薄膜瓶中。神经祖细胞标志物的表达,包括巢蛋白和性别决定Y盒2(SOX-2)，使用实时聚合酶链反应(RT-PCR)进行分析。使用Y迷宫检查大鼠的认知和功能记忆。使用SPSS软件(版本26.0)用单向方差分析(ANOVA)检验分析数据。
■巢蛋白的基因表达（29.74±0.42），SOX-2(31.44±0.67),与阳性对照组相比，AlCl3BM-MSCs组的Y-迷宫交替百分比（67.04±2.28）增加。RT-PCR分析表明,与阳性对照组相比,AlCl3+BM-MSCs组的巢蛋白(P<0.001)和SOX-2(P<0.001)显著增强。与阳性对照组相比,该组还表明AlCl3+BM-MSC组中Y-迷宫的交替百分比增加(P<0.001)。
■由于其对细胞治疗的潜在影响，在AD大鼠模型中发现BM-MSCs对大鼠行为损害和神经祖细胞标志物表达增加有效。
■这项研究于2022年在生物医学实验室医学院进行，安达拉斯大学，印度尼西亚。成年雄性Wistar大鼠（三组：阴性对照；无干预+PBS处理；阳性对照：AlCl3+水处理；AlCl3+BM-MSCs：AlCl3+BM-MSCs处理，每个n=5)每天口服AlCl3治疗五天。将干细胞以1×106个细胞/大鼠的剂量腹膜内注射到大鼠中。对照组给予相同量的磷酸盐缓冲盐水。干细胞注射后一个月,取出大鼠脑组织，并将其放入已制作的薄膜瓶中。神经祖细胞标志物的表达,包括巢蛋白和性别决定Y盒2(SOX-2)，使用实时聚合酶链反应(RT-PCR)进行分析。使用Y迷宫检查大鼠的认知和功能记忆。使用SPSS软件(版本26.0)用单向方差分析(ANOVA)检验分析数据。
■巢蛋白的基因表达（29.74±0.42），SOX-2(31.44±0.67),与阳性对照组相比，AlCl3BM-MSCs组的Y-迷宫交替百分比（67.04±2.28）增加。RT-PCR分析表明,与阳性对照组相比,AlCl3+BM-MSCs组的巢蛋白(P<0.001)和SOX-2(P<0.001)显著增强。与阳性对照组相比,该组还表明AlCl3+BM-MSC组中Y-迷宫的交替百分比增加(P<0.001)。
■由于其对细胞治疗的潜在影响，在AD大鼠模型中发现BM-MSCs对大鼠行为损害和神经祖细胞标志物表达增加有效。
