RESULTS: Inoculation effectively increased PAH removal in the short-term, but not in the long-term polluted soil. A decrease in the relative abundance of the inoculated genera was observed already on day 15 in the long-term polluted soil, while growth of these genera was observed in the short-term polluted soil, indicating establishment of the inoculum. In both soils, eukaryotic genera dominated as early incorporators of 13C-labelled biomass, while bacteria incorporated the labelled biomass at the end of the incubation period, probably through cross-feeding. We also found different successional patterns between the two soils. In the short-term polluted soil, Cercozoa and Fungi genera predominated as early incorporators, whereas Ciliophora, Ochrophyta and Amoebozoa were the predominant genera in the long-term polluted soil.
CONCLUSIONS: Our results showed differences in the inoculum establishment and predator community responses, affecting bioaugmentation efficiency. This highlights the need to further study predation effects on inoculum survival to increase the applicability of inoculation-based technologies. Video Abstract.
结果:接种在短期内有效地提高了PAH的去除,但不是在长期污染的土壤中。在长期污染的土壤中,第15天已经观察到接种属的相对丰度下降,虽然在短期污染的土壤中观察到这些属的生长,表明接种物的建立。在这两种土壤中,真核细胞属主要是13C标记生物质的早期掺入物,而细菌在培育期结束时掺入了标记的生物质,可能是通过交叉喂食。我们还发现两种土壤之间的演替模式不同。在短期污染的土壤中,Cercozoa和真菌属以早期掺入者为主,而Ciliophora,在长期污染的土壤中,Ochrophyta和Amoebozoa是主要属。
结论:我们的结果显示了接种物建立和捕食者群落反应的差异,影响生物强化效率。这凸显了需要进一步研究捕食对接种物存活的影响,以增加基于接种的技术的适用性。视频摘要。