PDF(Pubmed)
Abstract：
Iron-binding proteins, known as ferritins, play pivotal roles in immunological response, detoxification, and iron storage. Despite their significance to organisms, little is known about how they affect the immunological system of the red swamp crayfish (Procambarus clarkii). In our previous research, one ferritin subunit was completely discovered as an H-like subunit (PcFeH) from P. clarkii. The full-length cDNA of PcFerH is 1779 bp, including a 5\'-UTR (untranslated region, UTR) of 89 bp, 3\'-UTR (untranslated region, UTR) of 1180 bp and an ORF (open reading frame, ORF) of 510 bp encoding a polypeptide of 169 amino acids that contains a signal peptide and a Ferritin domain. The deduced PcFerH protein sequence has highly identity with other crayfish. PcFerH protein\'s estimated tertiary structure is quite comparable to animal structure. The PcFerH is close to Cherax quadricarinatus, according to phylogenetic analysis. All the organs examined showed widespread expression of PcFerH mRNA, with the ovary exhibiting the highest levels of expression. Additionally, in crayfish muscles, intestines, and gills, the mRNA transcript of PcFerH was noticeably up-regulated, after LPS and Poly I:C challenge. The expression of downstream genes in the immunological signaling system was suppressed when the PcFerH gene was knocked down. All of these findings suggested that PcFerH played a vital role in regulating the expression of downstream effectors in the immunological signaling pathway of crayfish.
摘要：
铁结合蛋白,被称为铁蛋白，在免疫反应中起关键作用，排毒,铁储存。尽管它们对生物体很重要，关于它们如何影响红色沼泽小龙虾（Procambarusclarkii）的免疫系统知之甚少。在我们之前的研究中,从克氏疟原虫中完全发现了一个铁蛋白亚基作为H样亚基（PcFeH）。PcFerH的cDNA全长为1779bp,包括5'-UTR(未翻译区域，UTR)为89bp，3\'-UTR(未翻译区域,UTR)1180bp和一个ORF(开放阅读框，510bp的ORF），编码169个氨基酸的多肽，该多肽包含信号肽和铁蛋白结构域。推导的PcFerH蛋白序列与其他小龙虾具有高度同一性。PcFerH蛋白的估计三级结构与动物结构相当。PcFerH靠近Cheraxquadricarinatus，根据系统发育分析。所有检查的器官均显示PcFerHmRNA的广泛表达，卵巢表现出最高的表达水平。此外,在小龙虾的肌肉中，肠子,和ill，PcFerH的mRNA转录物明显上调，在LPS和PolyI：C挑战后。当PcFerH基因敲低时，免疫信号系统中下游基因的表达受到抑制。所有这些发现表明，PcFerH在调节小龙虾免疫信号通路下游效应子的表达中起着至关重要的作用。
