Abstract：
UNASSIGNED: The subject of this article is the role of forensic toxicology in post-mortem examinations using immunofluorescence methods, its implications and its role in providing conclusive evidence for both criminal and civil proceedings. The aim of the study is to verify the correlation between the mode of death and the ingestion of exogenous substances and, if positive, to identify the category of substances ingested and assess their role in the cause of death.
UNASSIGNED: A laboratory study was carried out, consisting of several phases: pre-analytical phase; analytical phase; post-analytical phase. The variables analyzed were sex, cause of death, age. Abused substances tested: amphetamines, methamphetamines, barbiturates, benzodiazepines, cocaine, methadone, opiates, tricyclic antidepressants, delta-9-tetrahydrocannabinol (cannabis), alcohol.
UNASSIGNED: Retrospective analysis was performed on a total sample of 55 cases. The most relevant data emerged: cocaine with an incidence of 7.3% (4 cases out of 55), amphetamines with 5.4% (3 cases in total). The results of the screening tests were then subjected to confirmatory tests. There is an association between the use of certain exogenous substances and an increased risk of certain causes of death, such as overdose, traffic accidents, cardiovascular deaths, etc. This paper has highlighted the possibility of using first level immunological tests, such as immunofluorescence, to provide preliminary answers to the judicial authority immediately after autopsy, and a quantitative deepening with further second level tests, such as gas chromatography, as a gold standard to determine the cause of death.
UNASSIGNED: A laboratory study was carried out, consisting of several phases: pre-analytical phase; analytical phase; post-analytical phase. The variables analyzed were sex, cause of death, age. Abused substances tested: amphetamines, methamphetamines, barbiturates, benzodiazepines, cocaine, methadone, opiates, tricyclic antidepressants, delta-9-tetrahydrocannabinol (cannabis), alcohol.
UNASSIGNED: Retrospective analysis was performed on a total sample of 55 cases. The most relevant data emerged: cocaine with an incidence of 7.3% (4 cases out of 55), amphetamines with 5.4% (3 cases in total). The results of the screening tests were then subjected to confirmatory tests. There is an association between the use of certain exogenous substances and an increased risk of certain causes of death, such as overdose, traffic accidents, cardiovascular deaths, etc. This paper has highlighted the possibility of using first level immunological tests, such as immunofluorescence, to provide preliminary answers to the judicial authority immediately after autopsy, and a quantitative deepening with further second level tests, such as gas chromatography, as a gold standard to determine the cause of death.
摘要：
■本文的主题是法医毒理学在使用免疫荧光方法进行验尸中的作用，其含义及其在为刑事和民事诉讼提供确凿证据方面的作用。该研究的目的是验证死亡方式与摄入外源性物质之间的相关性，如果是积极的，确定摄入的物质类别并评估其在死亡原因中的作用。
■进行了一项实验室研究，由几个阶段组成:前分析阶段;分析阶段;后分析阶段。分析的变量是性别，死因,年龄。测试的滥用物质:苯丙胺，甲基苯丙胺,巴比妥酸盐,苯二氮卓类药物,可卡因,美沙酮,阿片类药物,三环抗抑郁药，delta-9-四氢大麻酚（大麻），酒精。
■对55例患者进行回顾性分析。最相关的数据出现了：可卡因发病率为7.3%（55例中有4例），含有5.4%的安非他明(共3例)。然后对筛选测试的结果进行确认测试。使用某些外源性物质与某些死亡原因的风险增加之间存在关联,比如用药过量,交通事故,心血管死亡,等。本文强调了使用一级免疫学测试的可能性，如免疫荧光，尸检后立即向司法当局提供初步答案，并通过进一步的二级测试进行定量深化，如气相色谱，作为确定死因的黄金标准。
■进行了一项实验室研究，由几个阶段组成:前分析阶段;分析阶段;后分析阶段。分析的变量是性别，死因,年龄。测试的滥用物质:苯丙胺，甲基苯丙胺,巴比妥酸盐,苯二氮卓类药物,可卡因,美沙酮,阿片类药物,三环抗抑郁药，delta-9-四氢大麻酚（大麻），酒精。
■对55例患者进行回顾性分析。最相关的数据出现了：可卡因发病率为7.3%（55例中有4例），含有5.4%的安非他明(共3例)。然后对筛选测试的结果进行确认测试。使用某些外源性物质与某些死亡原因的风险增加之间存在关联,比如用药过量,交通事故,心血管死亡,等。本文强调了使用一级免疫学测试的可能性，如免疫荧光，尸检后立即向司法当局提供初步答案，并通过进一步的二级测试进行定量深化，如气相色谱，作为确定死因的黄金标准。
