Abstract：
UNASSIGNED: This study aimed to evaluate an expanded matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF MS) database for the identification of Haemophilus species other than H. influenzae (Hi).
UNASSIGNED: A total of 144 Haemophilus species, cultured from respiratory samples from people (living) with cystic fibrosis, were identified with MALDI-TOF MS and 16S rRNA sequencing. Of these, 99 Haemophilus strains showed >99% similarity with the best matching strain in the National Center for Biotechnology Information (NCBI) database and were assigned to a single Haemophilus subspecies using both MALDI-TOF MS and 16S rRNA sequencing. The MS profiles of a subset of strains (n = 58/99) were added to the Bruker MALDI-TOF MS database. Subsequently, 270 different strains that were analyzed previously in a routine setting were re-analyzed.
UNASSIGNED: 16S rRNA sequencing reliably identified 99/144 Haemophilus strains (>99% similarity). H. haemolyticus 16S rRNA identification was suboptimal since only 3/21 H. haemolyticus strains attained a similarity of >99% with H. haemolyticus 16S rRNA sequence in the NCBI database. Expansion of the MALDI-TOF MS database improved the number of reliable identifications only moderately for H. haemolyticus, H. influenzae and H. paraphrohaemolyticus (<10%). By contrast, improved identification was more outspoken for H. parahaemolyticus, H. parainfluenzae, H. sputorum and H. pittmaniae (>85%).
UNASSIGNED: 16S rRNA sequencing is a valuable method for the identification of Haemophilus sp. other than Hi. Expansion of the MALDI-TOF MS database, based on 16S rRNA sequencing results, increased the proportion of reliable identifications and in this study resulted in an increase of 10% of Haemophilus sp. other than Hi strain identifications.
UNASSIGNED: A total of 144 Haemophilus species, cultured from respiratory samples from people (living) with cystic fibrosis, were identified with MALDI-TOF MS and 16S rRNA sequencing. Of these, 99 Haemophilus strains showed >99% similarity with the best matching strain in the National Center for Biotechnology Information (NCBI) database and were assigned to a single Haemophilus subspecies using both MALDI-TOF MS and 16S rRNA sequencing. The MS profiles of a subset of strains (n = 58/99) were added to the Bruker MALDI-TOF MS database. Subsequently, 270 different strains that were analyzed previously in a routine setting were re-analyzed.
UNASSIGNED: 16S rRNA sequencing reliably identified 99/144 Haemophilus strains (>99% similarity). H. haemolyticus 16S rRNA identification was suboptimal since only 3/21 H. haemolyticus strains attained a similarity of >99% with H. haemolyticus 16S rRNA sequence in the NCBI database. Expansion of the MALDI-TOF MS database improved the number of reliable identifications only moderately for H. haemolyticus, H. influenzae and H. paraphrohaemolyticus (<10%). By contrast, improved identification was more outspoken for H. parahaemolyticus, H. parainfluenzae, H. sputorum and H. pittmaniae (>85%).
UNASSIGNED: 16S rRNA sequencing is a valuable method for the identification of Haemophilus sp. other than Hi. Expansion of the MALDI-TOF MS database, based on 16S rRNA sequencing results, increased the proportion of reliable identifications and in this study resulted in an increase of 10% of Haemophilus sp. other than Hi strain identifications.
摘要：
■本研究旨在评估扩展的基质辅助激光解吸电离飞行时间质谱（MALDI-TOFMS）数据库，以鉴定流感嗜血杆菌（Hi）以外的嗜血杆菌。
■总共144种嗜血杆菌，从囊性纤维化患者（生活）的呼吸道样本中培养出来，用MALDI-TOFMS和16SrRNA测序鉴定。其中,99个嗜血杆菌菌株显示与国家生物技术信息中心(NCBI)数据库中的最佳匹配菌株>99%的相似性,并且使用MALDI-TOFMS和16SrRNA测序将其分配到单个嗜血杆菌亚种。将菌株子集(n=58/99)的MS谱添加到BrukerMALDI-TOFMS数据库中。随后，重新分析先前在常规设置中分析的270种不同菌株。
■16SrRNA测序可靠地鉴定了99/144个嗜血杆菌菌株（>99％相似性）。H.溶血杆菌16SrRNA鉴定是次优的,因为只有3/21H.溶血杆菌菌株与NCBI数据库中的溶血杆菌16SrRNA序列达到>99%的相似性。MALDI-TOFMS数据库的扩展仅适度地改善了溶血嗜血杆菌的可靠识别数量，流感嗜血杆菌和副嗜血杆菌（<10%）。相比之下,对副溶血H.H.副流感，H.putorum和H.pittmaniae（>85％）。
■16SrRNA测序是鉴定嗜血杆菌的一种有价值的方法。除了嗨。MALDI-TOFMS数据库的扩展，基于16SrRNA测序结果，增加了可靠鉴定的比例，在这项研究中，嗜血杆菌增加了10％。除了Hi菌株识别。
■总共144种嗜血杆菌，从囊性纤维化患者（生活）的呼吸道样本中培养出来，用MALDI-TOFMS和16SrRNA测序鉴定。其中,99个嗜血杆菌菌株显示与国家生物技术信息中心(NCBI)数据库中的最佳匹配菌株>99%的相似性,并且使用MALDI-TOFMS和16SrRNA测序将其分配到单个嗜血杆菌亚种。将菌株子集(n=58/99)的MS谱添加到BrukerMALDI-TOFMS数据库中。随后，重新分析先前在常规设置中分析的270种不同菌株。
■16SrRNA测序可靠地鉴定了99/144个嗜血杆菌菌株（>99％相似性）。H.溶血杆菌16SrRNA鉴定是次优的,因为只有3/21H.溶血杆菌菌株与NCBI数据库中的溶血杆菌16SrRNA序列达到>99%的相似性。MALDI-TOFMS数据库的扩展仅适度地改善了溶血嗜血杆菌的可靠识别数量，流感嗜血杆菌和副嗜血杆菌（<10%）。相比之下,对副溶血H.H.副流感，H.putorum和H.pittmaniae（>85％）。
■16SrRNA测序是鉴定嗜血杆菌的一种有价值的方法。除了嗨。MALDI-TOFMS数据库的扩展，基于16SrRNA测序结果，增加了可靠鉴定的比例，在这项研究中，嗜血杆菌增加了10％。除了Hi菌株识别。
