{Reference Type}: Journal Article
{Title}: Expansion of MALDI-TOF MS database as a strategy for identification of Haemophilus species other than H. influenzae.
{Author}: Willems E;Hamerlinck H;Messiaen AS;Schelstraete P;Van Braeckel E;Vande Weygaerde Y;Verhasselt B;Boelens J;Vandendriessche S;
{Journal}: Acta Clin Belg
{Volume}: 0
{Issue}: 0
{Year}: 2024 Aug 5
{Factor}: 1.682
{DOI}: 10.1080/17843286.2024.2386216
{Abstract}: <B>UNASSIGNED: </B>This study aimed to evaluate an expanded matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF MS) database for the identification of Haemophilus species other than H. influenzae (Hi).<BR><B>UNASSIGNED: </B>A total of 144 Haemophilus species, cultured from respiratory samples from people (living) with cystic fibrosis, were identified with MALDI-TOF MS and 16S rRNA sequencing. Of these, 99 Haemophilus strains showed >99% similarity with the best matching strain in the National Center for Biotechnology Information (NCBI) database and were assigned to a single Haemophilus subspecies using both MALDI-TOF MS and 16S rRNA sequencing. The MS profiles of a subset of strains (n = 58/99) were added to the Bruker MALDI-TOF MS database. Subsequently, 270 different strains that were analyzed previously in a routine setting were re-analyzed.<BR><B>UNASSIGNED: </B>16S rRNA sequencing reliably identified 99/144 Haemophilus strains (>99% similarity). H. haemolyticus 16S rRNA identification was suboptimal since only 3/21 H. haemolyticus strains attained a similarity of >99% with H. haemolyticus 16S rRNA sequence in the NCBI database. Expansion of the MALDI-TOF MS database improved the number of reliable identifications only moderately for H. haemolyticus, H. influenzae and H. paraphrohaemolyticus (<10%). By contrast, improved identification was more outspoken for H. parahaemolyticus, H. parainfluenzae, H. sputorum and H. pittmaniae (>85%).<BR><B>UNASSIGNED: </B>16S rRNA sequencing is a valuable method for the identification of Haemophilus sp. other than Hi. Expansion of the MALDI-TOF MS database, based on 16S rRNA sequencing results, increased the proportion of reliable identifications and in this study resulted in an increase of 10% of Haemophilus sp. other than Hi strain identifications.