METHODS: We retrospectively reviewed the results of comprehensive multiplex polymerase chain reaction (PCR) testing from November 2020 to March 2023 to estimate respiratory pathogen co-detection rates in Shinjuku, Tokyo. We evaluated the interactions of respiratory pathogens, particularly SARS-CoV-2, between observed and expected co-detection. We estimated the trend of co-detection with SARS-CoV-2 in terms of age and sex and applied a multiple logistic regression model adjusted for age, testing period, and sex to identify influencing factors between co-detection and single detection for each pathogen.
RESULTS: Among 57,746 patients who underwent multiplex PCR testing, 10,516 (18.2%) had positive for at least one of the 22 pathogens. Additionally, 881 (1.5%) patients were confirmed to have a co-detection. SARS-CoV-2 exhibited negative interactions with adenovirus, coronavirus, human metapneumovirus, parainfluenza virus, respiratory syncytial virus, and rhino/enterovirus. SARS-CoV-2 co-detection with other pathogens occurred most frequently in patients of the youngest age group (0-4 years). A multiple logistic regression model indicated that younger age was the most influential factor for SARS-CoV-2 co-detection with other respiratory pathogens.
CONCLUSIONS: The study highlights the prevalence of SARS-CoV-2 co-detection with other respiratory pathogens in younger age groups, necessitating further exploration of the clinical implications and severity of SARS-CoV-2 co-detection.
方法:我们回顾性回顾了2020年11月至2023年3月的综合多重聚合酶链反应(PCR)检测结果,以估计新宿的呼吸道病原体共检率,东京。我们评估了呼吸道病原体的相互作用,特别是SARS-CoV-2,在观察到的和预期的共同检测之间。我们估计了SARS-CoV-2在年龄和性别方面的共同检测趋势,并应用了调整年龄的多元逻辑回归模型,测试期,和性别,以确定每种病原体的共检测和单一检测之间的影响因素。
结果:在57,746名接受多重PCR检测的患者中,10,516(18.2%)对22种病原体中的至少一种呈阳性。此外,881例(1.5%)患者被证实有共同检测。SARS-CoV-2表现出与腺病毒的负相互作用,冠状病毒,人类偏肺病毒,副流感病毒,呼吸道合胞病毒,和犀牛/肠道病毒。SARS-CoV-2与其他病原体的共检测最常见于最年轻年龄组(0-4岁)的患者。多元logistic回归模型表明年龄较小是SARS-CoV-2与其他呼吸道病原体共检出的影响因素。
结论:该研究强调了年轻年龄组中SARS-CoV-2与其他呼吸道病原体的共同检测的患病率,需要进一步探索SARS-CoV-2共检测的临床意义和严重程度。