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Abstract：
In this study, the transepithelial transport of bioactive peptides derived from faba bean flour gastrointestinal digestates was investigated, in vitro, using a Caco-2 and HT29-MTX-E12 coculture monolayer, in comparison to those of pea and soy. The profile of transported peptides was determined by mass spectrometry, and the residual antioxidant activity was assessed. The ORAC value significantly (p < 0.05) decreased after transepithelial transport (24-36% reduction) for all legumes, while the antioxidant activity in ABTS assay significantly (p < 0.05) increased, as shown by the EC50 decrease of 26-44%. Five of the nine faba bean peptides that crossed the intestinal cell monolayer exhibited antioxidant activity. Two of these peptides, TETWNPNHPEL and TETWNPNHPE, were further hydrolyzed by the cells\' brush border peptidases to smaller fragments TETWNPNHP and TWNPNHPE. These metabolized peptides were synthesized, and both maintained high antioxidant activity in both ABTS (EC50 of 1.2 ± 0.2 and 0.4 ± 0.1 mM, respectively) and ORAC (2.5 ± 0.1 and 3.4 ± 0.2 mM of Trolox equivalent/mM, respectively) assays. These results demonstrated for the first time the bioaccessibility of faba bean peptides produced after in vitro gastrointestinal digestion and how their bioactive properties can be modulated during transepithelial transport.
摘要：
在这项研究中,研究了从蚕豆粉胃肠道消化物中获得的生物活性肽的跨上皮运输，在体外，使用Caco-2和HT29-MTX-E12共培养单层，与豌豆和大豆相比。通过质谱测定转运肽的谱,并评估残余的抗氧化活性。所有豆科植物的ORAC值经上皮运输后显著(p<0.05)下降(减少24-36%),而ABTS实验中的抗氧化活性显着增加（p<0.05），如EC50下降26-44%所示。穿过肠细胞单层的9种蚕豆肽中的5种表现出抗氧化活性。其中两种肽，TETWNPNHPEL和TETWNPNHPE，被细胞刷边界肽酶进一步水解成较小的片段TETWNPNHP和TWNPNHPE。这些代谢肽被合成，并且在两个ABTS中均保持高抗氧化活性（EC50为1.2±0.2和0.4±0.1mM，分别）和ORAC（2.5±0.1和3.4±0.2mM的Trolox当量/mM，分别)测定。这些结果首次证明了体外胃肠消化后产生的蚕豆肽的生物可及性，以及如何在跨上皮运输过程中调节其生物活性。
