Abstract：
Heart failure (HF) is a clinical syndrome caused by the progression of various cardiac diseases to severe stages, and exercise training plays a positive role in the development of HF. This study aimed to investigate the impact of different intensities of exercise training on HF rats.In this study, we established two HF rat models by intraperitoneal injection of isoproterenol at 2.5 mg/kg/day and abdominal aortic coarctation. After exercise training for 4 weeks, the heart weight/body weight ratio and echocardiography results were measured. Moreover, the regulatory effect of different exercise intensities on myocardial function in HF model rats was verified using tissue staining, western blotting, and reagent kits.Exercise training had a bidirectional adjust effect on HF. A running training program of 20 minutes/time had the most significant effect on improving myocardial function in HF rats, whereas exercise intensity of 40 minutes/time or 50 minutes/time did not significantly improve myocardial function in HF rats. Moreover, exercise intensities of 20 minutes/time and 30 minutes/time could reduce the expression levels of the HF markers NT-proBNP and BNP in rats, but the effect was more significant at a duration of 20 minutes/time. We also found that compared with other exercise intensities, 20 minutes/time exercise intensity could significantly improve myocardial fibrosis, promote cardiomyocyte autophagy, and reduce apoptosis in combating HF.Furthermore, an exercise intensity of 20 minutes/time can significantly ameliorate the progression of HF. However, the degree of significance of increasing exercise intensity in improving HF progression is weakened or has no significant effect.
摘要：
心力衰竭（HF）是由各种心脏病进展到严重阶段引起的临床综合征，运动训练对HF的发展起着积极的作用。本研究旨在探讨不同强度运动训练对HF大鼠的影响。在这项研究中,我们通过腹腔注射异丙肾上腺素2.5mg/kg/d和腹主动脉缩窄建立了两种HF大鼠模型。运动训练4周后,测量心脏重量/体重比和超声心动图结果。此外，用组织染色验证不同运动强度对HF模型大鼠心肌功能的调节作用,西方印迹,和试剂盒。运动训练对HF具有双向调节作用。20分钟/次的跑步训练对改善HF大鼠心肌功能的作用最显著,而40分钟/次或50分钟/次的运动强度并不能显著改善HF大鼠的心肌功能。此外，20分钟/次和30分钟/次的运动强度可降低大鼠HF标志物NT-proBNP和BNP的表达水平,但效果在20分钟/次的持续时间更显著。我们还发现，与其他运动强度相比，20分钟/次运动强度能明显改善心肌纤维化,促进心肌细胞自噬，在对抗HF时减少细胞凋亡。此外，20分钟/次的运动强度可以显着改善HF的进展。然而,增加运动强度对改善HF进展的重要性程度减弱或没有显着影响。
