Abstract：
Improper disposal of organic solid waste results in serious environmental pollution. Aerobic composting provides an environmentally friendly treatment method, but improving humification of raw materials remains a challenge. This study revealed the effect of different concentrations of potassium persulfate (PP) on humification of chicken manure and straw aerobic composting and the underlying microbial mechanisms. The results showed that when 0.6 % PP was added (PPH group), humus and the degree of polymerization were 80.77 mg/g and 2.52, respectively, which were significantly higher than those in 0.3 % PP (PPL group). As the concentration of PP was increased, the composition of rare taxa (RT) changed and improved in evenness, while abundant taxa (AT) was unaffected. Additionally, the density (0.037), edges (3278), and average degree (15.21) in the co-occurrence network decreased compared to PPL, while the average path (4.021) and modularity increased in PPH. This resulted in facilitating the turnover of matter, information, and energy among the microbes. Interestingly, cooperative behavior between microorganisms during the maturation period (24-60 d) occurred in PPH, but competitive relationships dominated in PPL. Cooperative behavior was positively correlated with humus (p < 0.05). Because the indices, such as higher degree, betweenness centrality, eigenvector centrality, and closeness centrality of the AT, were located in the microbial network center compared to RT, they were unaffected by the concentration of PP. The abundance of carbohydrate and amino acid metabolic pathways, which play an important role in humification, were higher in PPH. These findings contribute to understanding the relative importance of composition, interactions, and metabolic functionality of RT and AT on humification during chicken manure and straw aerobic composting under different concentrations of PP, as well as provide a basic reference for use of various conditioning agents to promote humification of organic solid waste.
摘要：
有机固体废物处置不当会造成严重的环境污染。好氧堆肥提供了一种环保的处理方法，但是改善原材料的屈辱仍然是一个挑战。这项研究揭示了不同浓度的过硫酸钾（PP）对鸡粪和秸秆好氧堆肥的腐殖化作用及其潜在的微生物机制。结果表明,当添加0.6%PP(PPH组)时,腐殖质和聚合度分别为80.77mg/g和2.52，显著高于0.3%PP(PPL组)。随着PP浓度的增加，稀有分类群(RT)的组成变化并提高了均匀度，而丰富的分类群（AT）不受影响。此外,节点(420)，边缘(3278),与PPL相比，共现网络中的平均度(15.21)下降，而PPH的平均路径(4.021)和模块化增加。这导致促进了物质的周转，信息,微生物中的能量。有趣的是,在PPH中发生的成熟期(24-60d)微生物之间的合作行为，但竞争关系在PPL中占主导地位。合作行为与腐殖质呈正相关(p<0.05)。因为指数，例如更高的学位，中间性中心性，特征向量中心性，以及AT的紧密度中心，与RT相比，位于微生物网络中心，它们不受PP浓度的影响。丰富的碳水化合物和氨基酸代谢途径，在羞辱中起着重要作用，PPH较高。这些发现有助于理解成分的相对重要性，互动,不同PP浓度下，RT和AT对鸡粪和秸秆好氧堆肥过程中腐殖质的代谢功能，以及为使用各种调理剂促进有机固体废物的腐殖化提供基本参考。
