Abstract：
Fish are currently used models for the toxicity assessment of chemicals, including polycyclic aromatic hydrocarbons (PAHs). Alternative methods including fish cell lines are currently used to provide fast and reliable results on the toxic properties of chemicals while respecting ethical concerns about animal testing. The Rainbow trout liver cell line RTLW1 was used to analyze the effects of two water-accommodated fractions from two crude oils: Arabian Light crude oil (LO) and refined oil from Erika (HO). Several toxicity endpoints were assessed in this study, including cytotoxicity, EROD activity, DNA damage (comet and micronucleus assays), and ROS production. RTL-W1 cells were exposed for 24 h at two or three dilutions of WAF at 1000 µg/L (0.1% (1 μg/L), 1% (10 μg/L), and 10% (100 μg/L)) for cytotoxicity and EROD activity and 1% and 10% for ROS production and genotoxicity). Exposure of RTL-W1 cells to LO WAF induced a significant increase of EROD activity and ROS production and altered DNA integrity as revealed by both the comet assay and the micronucleus test for 10 µg/L of LO. On the other hand, HO WAF exhibited limited toxic effects except for an EROD induction for 1% WAF dilution. These results confirmed the usefulness of RTL-W1 cells for in vitro toxicological assessment of chemical mixtures.
摘要：
鱼类是目前用于化学品毒性评估的模型,包括多环芳烃(PAHs)。目前使用包括鱼细胞系的替代方法来提供关于化学品毒性特性的快速和可靠的结果,同时尊重对动物试验的伦理关注。虹鳟鱼肝细胞系RTLW1用于分析来自两种原油的两种水容纳馏分的影响：阿拉伯轻质原油（LO）和来自Erika（HO）的精制油。在这项研究中评估了几个毒性终点，包括细胞毒性，EROD活动,DNA损伤（彗星和微核试验），和ROS生产。RTL-W1细胞在1000µg/L（0.1％（1μg/L）的WAF的两个或三个稀释液中暴露24小时，1%(10μg/L)，和10％（100μg/L））的细胞毒性和EROD活性以及1％和10％的ROS产生和遗传毒性）。如彗星测定法和10µg/LLO的微核试验所揭示的，将RTL-W1细胞暴露于LOWAF会导致EROD活性和ROS产生显着增加，并改变DNA完整性。另一方面,除了1％WAF稀释的EROD诱导外，HOWAF表现出有限的毒性作用。这些结果证实了RTL-W1细胞用于化学混合物的体外毒理学评估的有用性。
