PDF(Pubmed)
Abstract：
BACKGROUND: Topical corticosteroids treat cutaneous inflammation but have side effects. In earlier studies, bilirubin exhibited anti-inflammatory effect, but its hydrophobicity and poor absorption limit its potential.
OBJECTIVE: Synthesis of bilirubin nanoparticles (BNP) and bilirubin nanoparticles gels (BNP gel) to study the anti-inflammatory effect of topical BNP gel against carrageenan-induced rat paw edema in Wistar rats.
METHODS: BNP were synthesized, and BNP gels were prepared by mixing BNP of different concentrations with pluronic F-127 (PF-127). A different group for each formulation was assigned with five rats in each group. After 1 h of carrageenan (1% [w/v]) injection in each group, different gels were applied topically to their respective groups. Paw edema size, percent inflammation, percent edema inhibition, and inhibition time50 were evaluated. Interleukin-10 (IL-10) levels and neutrophil infiltration in rat paw tissue were evaluated by enzyme-linked immunosorbent assay and hematoxylin and eosin, respectively.
RESULTS: Synthesized spherical-shaped BNP had negative zeta potential. BNP gels markedly reduced paw edema size and % inflammation as compared to carrageenan and bulk bilirubin gel (Bulk B gel) treated group and significantly increased IL-10 levels and inhibited neutrophil infiltration.
CONCLUSIONS: BNP gels exhibited a better anti-inflammatory effect than bulk B gel and comparable anti-inflammatory potential with clobetasol.
OBJECTIVE: Synthesis of bilirubin nanoparticles (BNP) and bilirubin nanoparticles gels (BNP gel) to study the anti-inflammatory effect of topical BNP gel against carrageenan-induced rat paw edema in Wistar rats.
METHODS: BNP were synthesized, and BNP gels were prepared by mixing BNP of different concentrations with pluronic F-127 (PF-127). A different group for each formulation was assigned with five rats in each group. After 1 h of carrageenan (1% [w/v]) injection in each group, different gels were applied topically to their respective groups. Paw edema size, percent inflammation, percent edema inhibition, and inhibition time50 were evaluated. Interleukin-10 (IL-10) levels and neutrophil infiltration in rat paw tissue were evaluated by enzyme-linked immunosorbent assay and hematoxylin and eosin, respectively.
RESULTS: Synthesized spherical-shaped BNP had negative zeta potential. BNP gels markedly reduced paw edema size and % inflammation as compared to carrageenan and bulk bilirubin gel (Bulk B gel) treated group and significantly increased IL-10 levels and inhibited neutrophil infiltration.
CONCLUSIONS: BNP gels exhibited a better anti-inflammatory effect than bulk B gel and comparable anti-inflammatory potential with clobetasol.
摘要：
背景：外用糖皮质激素治疗皮肤炎症，但有副作用。在早期的研究中，胆红素表现出抗炎作用，但其疏水性和吸收性差限制了其潜力。
目的：合成胆红素纳米颗粒（BNP）和胆红素纳米颗粒凝胶（BNP凝胶），研究局部用BNP凝胶对角叉菜胶诱导的Wistar大鼠爪水肿的抗炎作用。
方法：合成BNP，通过将不同浓度的BNP与pluronicF-127(PF-127)混合来制备BNP凝胶。每种制剂的不同组被分配为每组五只大鼠。每组注射角叉菜胶(1%[w/v])1小时后，将不同的凝胶局部应用于它们各自的组。爪水肿大小,百分比炎症，水肿抑制百分比，和抑制时间50进行评估。通过酶联免疫吸附试验和苏木精、伊红评价大鼠爪组织中白细胞介素-10(IL-10)水平和中性粒细胞浸润,分别。
结果：合成的球形BNP具有负zeta电位。与角叉菜胶和散装胆红素凝胶（BulkB凝胶）治疗组相比，BNP凝胶显着降低了爪水肿的大小和炎症百分比，并显着增加了IL-10水平并抑制了中性粒细胞浸润。
结论：BNP凝胶显示出比散装B凝胶更好的抗炎作用，并且与氯倍他索具有相当的抗炎潜力。
目的：合成胆红素纳米颗粒（BNP）和胆红素纳米颗粒凝胶（BNP凝胶），研究局部用BNP凝胶对角叉菜胶诱导的Wistar大鼠爪水肿的抗炎作用。
方法：合成BNP，通过将不同浓度的BNP与pluronicF-127(PF-127)混合来制备BNP凝胶。每种制剂的不同组被分配为每组五只大鼠。每组注射角叉菜胶(1%[w/v])1小时后，将不同的凝胶局部应用于它们各自的组。爪水肿大小,百分比炎症，水肿抑制百分比，和抑制时间50进行评估。通过酶联免疫吸附试验和苏木精、伊红评价大鼠爪组织中白细胞介素-10(IL-10)水平和中性粒细胞浸润,分别。
结果：合成的球形BNP具有负zeta电位。与角叉菜胶和散装胆红素凝胶（BulkB凝胶）治疗组相比，BNP凝胶显着降低了爪水肿的大小和炎症百分比，并显着增加了IL-10水平并抑制了中性粒细胞浸润。
结论：BNP凝胶显示出比散装B凝胶更好的抗炎作用，并且与氯倍他索具有相当的抗炎潜力。
