Abstract：
In this study, we investigated the effects of flickering light on refractive development of mice and the changes of fundus structure and function during this process. C57BL/6 mice were randomly divided into control group and flickering light-induced myopia (FLM) group. Mice in the control group were fed under normal lighting. FLM group mice were fed under lighting with a duty cycle of 50% and flash frequency of 2 Hz. Refractive status, axial length (AL), corneal radius of curvature (CRC), and electroretinogram signals were measured in all animals before treatment and at 2 and 4 weeks after treatment. Retinal thickness (RT), choroidal thickness (ChT) and choroidal blood perfusion (ChBP) were measured by optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA). After 4 weeks of flickering light stimulation, the mice became myopia, the AL increased, but the CRC remained constant. The induction of myopia reduced the implicit time and amplitude of a-wave and b-wave in electroretinogram, which affects the function of retina. Full-layer retinal thickness, ChT and ChBP decreased at both 2 and 4 weeks after flickering light induction. The superficial and middle layers of the retina were significantly thinner, while the deep layer was only slightly thinner without statistical significance. Calculated by the concentric circle algorithm, the decrease of choroidal blood perfusion in FLM was mainly concentrated in the concentric circle area with the optic disc as the center radius of 150-450 μm. In conclusion, the present study shows that flickering light can successfully induce myopia in C57BL/6 mice, affect the electrophysiological activity of retina, and cause changes in fundus tissue structure and blood flow.
摘要：
在这项研究中,我们研究了闪烁光对小鼠屈光发育的影响以及在此过程中眼底结构和功能的变化。将C57BL/6小鼠随机分为对照组和闪烁光诱导近视(FLM)组。对照组小鼠在正常光照下喂养。FLM组小鼠在50%占空比和2Hz闪光频率的照明下喂养。折射状态,轴向长度（AL），角膜曲率半径（CRC），在治疗前和治疗后2周和4周测量所有动物的视网膜电图信号。视网膜厚度（RT），通过光学相干断层扫描（OCT）和光学相干断层扫描血管造影（OCTA）测量脉络膜厚度（ChT）和脉络膜血液灌注（ChBP）。经过4周的闪烁光刺激，老鼠变成了近视，AL增加，但CRC保持不变。近视的诱导降低了视网膜电图中a波和b波的隐含时间和振幅,影响视网膜的功能。全层视网膜厚度,闪烁光诱导后2周和4周时，ChT和ChBP均降低。视网膜的表层和中间层明显变薄，而深层仅略薄，无统计学意义。由同心圆算法计算，FLM脉络膜血流灌注的减少主要集中在以视盘为中心半径150-450μm的同心圆区域。总之,本研究表明，闪烁光可以成功诱导C57BL/6小鼠近视，影响视网膜的电生理活动，并引起眼底组织结构和血流的变化。
