PDF(Pubmed)
Abstract：
The proficient handling of diabetic wounds, a rising issue coinciding with the global escalation of diabetes cases, poses significant clinical difficulties. A range of biofunctional dressings have been engineered and produced to expedite the healing process of diabetic wounds. This study proposes a multifunctional hydrogel dressing for diabetic wound healing, which is composed of Polyvinyl Alcohol (PVA) and N1-(4-boronobenzyl)-N3-(4-boronophenyl)-N1, N1, N3, N3-teramethylpropane-1, 3-diaminium (TSPBA), and a dual-drug loaded Gelatin methacryloyl (GM) microgel. The GM microgel is loaded with sodium fusidate (SF) and nanoliposomes (LP) that contain metformin hydrochloride (MH). Notably, adhesive and self-healing properties the hydrogel enhance their therapeutic potential and ease of application. In vitro assessments indicate that SF-infused hydrogel can eliminate more than 98% of bacteria within 24 h and maintain a sustained release over 15 days. Additionally, MH incorporated within the hydrogel has demonstrated effective glucose level regulation for a duration exceeding 15 days. The hydrogel demonstrates a sustained ability to neutralize ROS throughout the entire healing process, predominantly by electron donation and sequestration. This multifunctional hydrogel dressing, which integrated biological functions of efficient bactericidal activity against both MSSA and MRSA strains, blood glucose modulation, and control of active oxygen levels, has successfully promoted the healing of diabetic wounds in rats in 14 days. The hydrogel dressing exhibited significant effectiveness in facilitating the healing process of diabetic wounds, highlighting its considerable promise for clinical translation.
摘要：
熟练处理糖尿病伤口，随着糖尿病病例的全球升级，造成重大的临床困难。已经设计并生产了一系列生物功能敷料以加快糖尿病伤口的愈合过程。这项研究提出了一种用于糖尿病伤口愈合的多功能水凝胶敷料，由聚乙烯醇（PVA）和N1-（4-硼苄基）-N3-（4-硼苯基）-N1，N1，N3，N3-三甲基丙烷-1，3-二胺（TSPBA）组成，和双载药明胶甲基丙烯酰基(GM)微凝胶。GM微凝胶装载有夫西酸钠(SF)和含有盐酸二甲双胍(MH)的纳米脂质体(LP)。值得注意的是,水凝胶的粘附性和自修复性增强了它们的治疗潜力和易于应用。体外评估表明，注入SF的水凝胶可以在24小时内消除98％以上的细菌，并在15天内保持持续释放。此外,在超过15天的持续时间内,在水凝胶内掺入的MH已经证明了有效的葡萄糖水平调节。水凝胶在整个愈合过程中表现出中和ROS的持续能力，主要通过电子捐赠和隔离。这种多功能水凝胶敷料，整合了对MSSA和MRSA菌株的有效杀菌活性的生物学功能，血糖调节，控制活性氧水平，在14天内成功促进了大鼠糖尿病伤口的愈合。水凝胶敷料在促进糖尿病伤口的愈合过程中表现出显著的有效性，突出了其临床翻译的巨大前景。
