Abstract：
Phloem-specific promoter efficiently triggers graft-transmissible RNA interference (gtRNAi). We leveraged a phloem-specific promoter derived from the Rice tungro bacilliform virus, optimizing the RNAi mechanism\'s efficiency and specificity. Here, we detail the construction of phloem-specific promoter-based gtRNAi system and its application through grafting experiments, demonstrating its effectiveness in inducing tomato yellow leaf curl Thailand virus (TYLCHTV) resistance in non-transgenic scions. This strategy presents a practical application for protecting crops against viruses without genetically modifying the entire plant.
摘要：
韧皮部特异性启动子有效触发移植物可传输的RNA干扰(gtRNAi)。我们利用了来自Ricetungro杆状病毒的韧皮部特异性启动子，优化RNAi机制的效率和特异性。这里,我们通过嫁接实验详细介绍了基于韧皮部特异性启动子的gtRNAi系统的构建及其应用,证明了其在非转基因接穗中诱导番茄黄叶卷曲泰国病毒（TYLCHTV）抗性的有效性。该策略提供了在不对整个植物进行遗传修饰的情况下保护作物免受病毒侵害的实际应用。
