Abstract：
Traditionally, hybrid promoters are constructed, in Saccharomyces cerevisiae, by joining the core region and the upstream activating sequences from different native promoters. Here, we describe a new design that makes use of the core promoters from foreign organisms: viruses, humans, and the yeast Schizosaccharomyces pombe. With this approach, we realized a library of 59 new constitutive promoters that span over nine folds in gene expression.
摘要：
传统上,构建了杂合启动子，在酿酒酵母中，通过连接核心区和来自不同天然启动子的上游激活序列。这里,我们描述了一种新的设计，利用外来生物的核心启动子：病毒，人类,和裂殖酵母。通过这种方法，我们实现了59个新的组成型启动子的文库，这些启动子在基因表达中跨越了9倍以上。
