%0 Journal Article
%T Hybrid Synthetic Promoters in Saccharomyces cerevisiae Built on Foreign Promoter Sequences.
%A Feng X
%A Marchisio MA
%J Methods Mol Biol
%V 2844
%N 0
%D 2024
%M 39068335
暂无%R 10.1007/978-1-0716-4063-0_7
%X Traditionally, hybrid promoters are constructed, in Saccharomyces cerevisiae, by joining the core region and the upstream activating sequences from different native promoters. Here, we describe a new design that makes use of the core promoters from foreign organisms: viruses, humans, and the yeast Schizosaccharomyces pombe. With this approach, we realized a library of 59 new constitutive promoters that span over nine folds in gene expression.