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Abstract：
Salivary glands\' neoplasms are hard to diagnose and present a complex etiology. However, several viruses have been detected in these neoplasms, such as HCMV, which can play a role in certain cancers through oncomodulation. The co-infections between HCMV with betaherpesviruses (HHV-6 and HHV-7) and polyomaviruses (JCV and BKV) has been investigated. The aim of the current study is to describe the frequency of HCMV and co-infections in patients presenting neoplastic and non-neoplastic lesions, including in the salivary gland. Multiplex quantitative polymerase chain reaction was used for betaherpesvirus and polyomavirus quantification purposes after DNA extraction. In total, 50.7% of the 67 analyzed samples were mucocele, 40.3% were adenoma pleomorphic, and 8.9% were mucoepidermoid carcinoma. Overall, 20.9% of samples presented triple-infections with HCMV/HHV-6/HHV-7, whereas 9.0% were co-infections with HCMV/HHV-6 and HCMV/HHV-7. The largest number of co-infections was detected in pleomorphic adenoma cases. All samples tested negative for polyomaviruses, such as BKV and JCV. It was possible to conclude that HCMV can be abundant in salivary gland lesions. A high viral load can be useful to help better understand the etiological role played by viruses in these lesions. A lack of JCV and BKV in the samples analyzed herein does not rule out the involvement of these viruses in one or more salivary gland lesion subtypes.
摘要：
唾液腺肿瘤难以诊断，病因复杂。然而,在这些肿瘤中检测到几种病毒，如HCMV，它可以通过调节在某些癌症中发挥作用。已经研究了HCMV与β疱疹病毒（HHV-6和HHV-7）和多瘤病毒（JCV和BKV）之间的共感染。本研究的目的是描述HCMV的频率和共同感染的患者呈现肿瘤和非肿瘤性病变，包括唾液腺。在DNA提取后，多重定量聚合酶链反应用于β疱疹病毒和多瘤病毒定量目的。总的来说,67个分析样本中50.7%为黏液囊肿,40.3%为多形性腺瘤，粘液表皮样癌占8.9%。总的来说,20.9％的样本出现HCMV/HHV-6/HHV-7的三重感染，而9.0％的样本出现HCMV/HHV-6和HCMV/HHV-7的共同感染。在多形性腺瘤病例中检测到最多的共感染。所有样本检测多瘤病毒呈阴性,例如BKV和JCV。可以得出结论，HCMV在唾液腺病变中可能很丰富。高病毒载量有助于更好地了解病毒在这些病变中的病因学作用。本文分析的样品中JCV和BKV的缺乏不排除这些病毒参与一种或多种唾液腺病变亚型。
