PDF(Pubmed)
Abstract：
Coccidiosis, a parasitic disease caused by single or multiple Eimeria species, leads to significant economic losses in the poultry industry. The Eimeria life cycle includes schizogony, gametogony, and sporogony. To investigate the dynamics of gene expression and regulatory networks during the development of Eimeria acervulina, we employed time-course transcriptomics to rigorously compare the gene expression patterns between a precocious line (PL) and the wild type (WT) of E. acervulina. The results revealed that the PL enters into gametogony 12 h earlier than the WT, and both the PL and WT exhibited distinct clustering patterns during the development phase. A weighted gene co-expression network analysis (WGCNA) identified genes specifically expressed at four distinct developmental stages, schizogony, gametogony, sporulated oocysts, and unsporulated oocysts, clarifying the key biological processes at each stage. This study used global transcriptome profiling to elucidate molecular variations throughout the E. acervulina life cycle, providing critical insights into molecular characterization and valuable resources for investigating other apicomplexan parasites of public health importance.
摘要：
球虫病,由单个或多个艾美球虫引起的寄生虫病，导致家禽业的重大经济损失。艾美球虫生命周期包括分裂，gametogony,和sposogony。为了研究艾美耳球虫发育过程中基因表达和调控网络的动态变化，我们采用时程转录组学方法严格比较了早熟大肠杆菌（PL）和野生型（WT）之间的基因表达模式。结果表明，PL比WT早12小时进入配子，PL和WT在开发阶段都表现出不同的聚类模式。加权基因共表达网络分析（WGCNA）确定了在四个不同发育阶段特异性表达的基因，分裂,gametogony,有孢子的卵囊,和无孢子的卵囊，阐明每个阶段的关键生物过程。这项研究使用全球转录组分析来阐明整个E.acervulina生命周期的分子变异，提供对分子表征的关键见解和宝贵的资源，以调查其他具有公共卫生重要性的尖丛寄生虫。
