PDF(Pubmed)
Abstract：
Guanidinoacetic acid (GAA) can effectively improve the metabolism of energy and proteins by stimulating creatine biosynthesis. We present a study exploring the impact of GAA on production performance, serum biochemistry, meat quality and rumen fermentation in Hu sheep. A total of 144 weaned male Hu sheep (body weight 16.91 ± 3.1 kg) were randomly assigned to four groups with three replicates of twelve sheep in each group. The diets were supplemented with 0 (CON), 500 (GAA-1), 750 (GAA-2) and 1000 mg/kg (GAA-3) of GAA (weight of feed), respectively. After a comprehensive 90-day experimental period, we discovered that the supplementation of GAA had a remarkable impact on various muscle parameters. Specifically, it significantly enhanced the average daily growth (ADG) of the animals and improved the shear force and fiber diameter of the muscle, while also reducing the drip loss and muscle fiber density. Furthermore, the addition of GAA to the feed notably elevated the serum concentrations of high-density lipoprotein cholesterol (HDL-C), total protein (TP) and globulin (GLB), as well as the enzyme activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Concurrently, there was a decrease in the levels of triglycerides (TG) and malondialdehyde (MDA) in the serum. In addition, GAA decreased the pH and the acetate-to-propionate ratio and increased the total volatile fatty acids (TVFA) and ammoniacal nitrogen (NH3-N) levels of rumen fluid. Additionally, GAA upregulated acetyl-CoA carboxylase (ACC) gene expression in the Hu sheep\'s muscles. In conclusion, our findings suggest that GAA supplementation not only enhances muscle quality but also positively affects serum biochemistry and ruminal metabolism, making it a potential candidate for improving the overall health and performance of Hu sheep.
摘要：
胍基乙酸（GAA）通过刺激肌酸的生物合成，可以有效地改善能量和蛋白质的代谢。我们提出了一项研究，探讨了GAA对生产性能的影响，血清生物化学,湖羊的肉品质和瘤胃发酵。将144只断奶雄性湖羊（体重16.91±3.1kg）随机分为4组，每组3只，每组12只。饮食中添加了0（CON），500(GAA-1),750（GAA-2）和1000mg/kg（GAA-3）的GAA（饲料重量），分别。经过90天的全面实验，我们发现补充GAA对各种肌肉参数有显著影响。具体来说,显着提高了动物的平均日生长（ADG），并改善了肌肉的剪切力和纤维直径，同时还减少了滴水损失和肌肉纤维密度。此外，在饲料中添加GAA显着升高了高密度脂蛋白胆固醇（HDL-C）的血清浓度，总蛋白（TP）和球蛋白（GLB），以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的酶活性。同时,血清中甘油三酯（TG）和丙二醛（MDA）水平降低。此外,GAA降低了pH值和乙酸盐与丙酸盐的比率，并增加了瘤胃液的总挥发性脂肪酸（TVFA）和氨态氮（NH3-N）水平。此外,GAA上调湖羊肌肉中乙酰辅酶A羧化酶（ACC）基因的表达。总之,我们的研究结果表明，补充GAA不仅可以提高肌肉质量，而且可以积极影响血清生化和瘤胃代谢，使其成为改善湖羊整体健康和性能的潜在候选人。
