Abstract：
Ultrasonic-assisted extraction using a natural deep eutectic solvent (UAE-NADES) is an efficient method for extracting grape seed polyphenols (GSPs). In this study, response surface methodology was used to optimize the extraction of GSPs with UAE-NADES, and the theoretical extraction rate of GSPs was 139.014 mg GAE/g, the actual extraction rate was 135.78 ± 1.3 mg GAE/g. A pseudo-second-order kinetic extraction fitting was established to simulate the extraction process and mechanism (R2 > 0.99). Analysis of antioxidant capacity, Fourier-infrared spectroscopy and scanning electron microscopy revealed that UAE-NADES works synergetically to maintain the stability of extracted GSPs. The results of high-performance liquid chromatography showed that catechin (41.14 mg/g) is the main component of GSPs in the extract. The UAE-NADES extraction of GSPs can inhibit the growth of Alternaria alternata at 0.25 mg GAE/g, while the GSPs extracted by other methods can effectively inhibit the growth of Alternaria alternata at 0.35 mg GAE/g. Thus, this study demonstrates that UAE-NADES is a high-efficiency means of extracting GSPs and, in a wider sense, is a promising extraction technology for the green utilization of waste resources.
摘要：
使用天然深共晶溶剂（UAE-NADES）的超声波辅助提取是提取葡萄籽多酚（GSPs）的有效方法。在这项研究中,采用响应面法优化UAE-NADES提取GSPs，GSPs的理论提取率为139.014mgGAE/g,实际提取率为135.78±1.3mgGAE/g。建立了拟二级动力学萃取拟合来模拟萃取过程和机理(R2>0.99)。抗氧化能力分析，傅里叶红外光谱和扫描电子显微镜显示，UAE-NADES协同作用以保持提取的GSP的稳定性。高效液相色谱结果表明，儿茶素（41.14mg/g）是提取物中GSPs的主要成分。UAE-NADES提取的GSPs在0.25mgGAE/g时可以抑制链格孢菌的生长，而其他方法提取的GSPs在0.35mgGAE/g时可有效抑制链格孢菌的生长。因此，这项研究表明，UAE-NADES是提取GSP的高效手段，在更广泛的意义上，是一种很有前途的废弃资源绿色利用提取技术。
