Abstract：
The recovery of bioactive compounds is a promising approach for obtaining rich extracts from fruit by-products. This study investigated the influence of Natural Deep Eutectic Solvents (NADES) and Ultrasound-Assisted Extraction (UAE) on the phenolic content, antioxidant capacity, and in vitro antidiabetic activity of Psidium myrtoides by-product. Among eight NADES evaluated based on choline chloride, NADES ChCl:Gly (1:2) was selected for its efficiency in extracting total phenolic compounds (TPC) with high antioxidant capacity. The optimized conditions were 61 °C, a solid-liquid ratio of 100 mg 5 mL-1, and a 60-minute extraction time. ChCl:Gly exhibited superior TPC recovery (2.6-fold greater effectiveness) compared to the 60 % hydroethanolic solution. Twenty-six phenolic compounds were identified, including significant levels of catechin (336.48 mg g-1) and isoquercetin (26.09 mg g-1). Phenolic acids, such as p-anisic acid (5.47 mg g-1) and methoxyphenylacetic acid (0.23 mg g-1), were identified for the first time in the purple araçá by-product. The ChCl:Gly extract demonstrated the highest bioactivity, showcasing antioxidant and antidiabetic capacities. This study introduces an innovative and sustainable alternative for recovering phenolic compounds from fruit by-products, offering enhanced recovery efficiency and/or selectivity compared to organic solvents.
摘要：
生物活性化合物的回收是从水果副产品中获得丰富提取物的有希望的方法。这项研究调查了天然深共晶溶剂（NADES）和超声辅助萃取（UAE）对酚类含量的影响，抗氧化能力，及其副产物的体外抗糖尿病活性。在基于氯化胆碱评估的八个NADES中，NADES选择ChCl：Gly（1：2），因为它具有高抗氧化能力的提取总酚类化合物（TPC）的效率。优化条件为61℃,固液比为100mg5mL-1，提取时间为60分钟。与60%的氢乙醇溶液相比,ChCl:Gly表现出优异的TPC回收率(高2.6倍的有效性)。确定了26种酚类化合物，包括显著水平的儿茶素(336.48mgg-1)和异槲皮素(26.09mgg-1)。酚酸,例如对茴香酸（5.47mgg-1）和甲氧基苯乙酸（0.23mgg-1），在紫色的阿拉卡亚副产品中首次被发现。ChCl：Gly提取物表现出最高的生物活性，展示抗氧化和抗糖尿病能力。这项研究介绍了一种创新和可持续的替代方法，用于从水果副产品中回收酚类化合物，提供与有机溶剂相比提高的回收效率和/或选择性。
