Abstract：
Elderly individuals undergoing surgical procedures are often confronted with the peril of experiencing postoperative cognitive dysfunction (POCD). Prior research has demonstrated the exacerbating effect of sevoflurane anesthesia on neuroinflammation, which can further deteriorate the condition of POCD in elderly patients. Intermittent fasting (IF) restricts food consumption to a specific time window and has been demonstrated to ameliorate cognitive dysfunction induced by neuropathic inflammation. We subjected 18-month-old male mice to 16 hours of fasting and 8 hours of unrestricted eating over a 24-hour period for 0, 1, 2, and 4 weeks, followed by abdominal exploration under sevoflurane anesthesia. In this study, we aim to explore the potential impact of IF on postoperative cognitive function in aged mice undergoing sevoflurane surgery through the preoperative implementation of IF measures. The findings indicate two weeks of IF leads to a significant enhancement of learning and memory capabilities in mice following surgery. The cognitive performance, as determined by the novel object recognition and Morris water maze tests, as well as the synaptic plasticity, as measured by in vivo electrophysiological recordings, has demonstrated marked improvements. Furthermore, the administration of IF markedly enhances the expression of synaptic-associated proteins in hippocampal neurons, concomitant with a decreasing expression of pro-inflammatory factors and a reduced density of microglial cells within the hippocampal brain region. To summarize, the results of this study indicate that IF may mitigate inflammation in the hippocampal area of the brain. Furthermore, IF appears to provide a safeguard against cognitive impairment and synaptic plasticity impairment brought on by sevoflurane anesthesia.
摘要：
接受外科手术的老年人经常面临术后认知功能障碍（POCD）的危险。先前的研究已经证明七氟醚麻醉对神经炎症的加重作用，这可以进一步恶化老年患者的POCD状况。间歇性禁食（IF）将食物消耗限制在特定的时间窗口，并已被证明可以改善由神经性炎症引起的认知功能障碍。我们对18个月大的雄性小鼠进行了16小时的禁食和8小时的无限制进食，持续0、1、2和4周，随后在七氟烷麻醉下进行腹部探查。在这项研究中,我们旨在通过术前实施IF措施,探讨IF对七氟醚手术老年小鼠术后认知功能的潜在影响.研究结果表明，两周的IF可以显着提高手术后小鼠的学习和记忆能力。认知表现，根据新颖的物体识别和莫里斯水迷宫测试确定，以及突触可塑性，通过体内电生理记录测量，已经证明了显著的改进。此外，给药IF能显著增强海马神经元突触相关蛋白的表达,伴随着促炎因子表达的减少和海马脑区小胶质细胞密度的降低。总结一下,这项研究的结果表明，IF可以减轻大脑海马区的炎症。此外，IF似乎可以防止七氟醚麻醉引起的认知障碍和突触可塑性障碍。
