Abstract：
In ART,1 optimized sperm preparation could separate spermatozoa from potentially harmful germs and cells, improving sperm parameters. This study compares the outcome of a routinely used two-layer density gradient with a three-layer protocol in terms of sperm and microbiological parameters. According to the results, a three-layer protocol was more effective.
摘要：
在艺术中,1优化的精子制备可以从潜在有害的细菌和细胞中分离精子，改善精子参数。这项研究比较了常规使用的两层密度梯度和三层方案在精子和微生物参数方面的结果。根据结果,三层协议更有效。
