{Reference Type}: Journal Article
{Title}: Optimization of the microbiological treatment of sperm for assisted reproductive technology (ART).
{Author}: Bekon AM;Martiny D;Thomas AL;Miendje Y;Debyttere AL;Autin C;Bertrand E;
{Journal}: J Microbiol Methods
{Volume}: 224
{Issue}: 0
{Year}: 2024 Sep 22
{Factor}: 2.622
{DOI}: 10.1016/j.mimet.2024.107004
{Abstract}: In ART,1 optimized sperm preparation could separate spermatozoa from potentially harmful germs and cells, improving sperm parameters. This study compares the outcome of a routinely used two-layer density gradient with a three-layer protocol in terms of sperm and microbiological parameters. According to the results, a three-layer protocol was more effective.