%0 Journal Article
%T Optimization of the microbiological treatment of sperm for assisted reproductive technology (ART).
%A Bekon AM
%A Martiny D
%A Thomas AL
%A Miendje Y
%A Debyttere AL
%A Autin C
%A Bertrand E
%J J Microbiol Methods
%V 224
%N 0
%D 2024 Sep 22
%M 39047945
%F 2.622
%R 10.1016/j.mimet.2024.107004
%X In ART,1 optimized sperm preparation could separate spermatozoa from potentially harmful germs and cells, improving sperm parameters. This study compares the outcome of a routinely used two-layer density gradient with a three-layer protocol in terms of sperm and microbiological parameters. According to the results, a three-layer protocol was more effective.