Abstract：
Listeria monocytogenes (Lm) is a pathogenic bacteria able to grow at refrigerated temperatures, widely distributed in the environment. This bacteria is susceptible to contaminate various food products of which refrigerated ready-to-eat foods (RTEF) may pose a risk for public health. In Europe, food business operators (FBOs) shall ensure that foodstuffs comply with the relevant microbiological criteria set out in the Regulation (EC) N°2073/2005. Food safety criteria for Lm are defined in RTEF throughout their shelf-life. FBOs should implement studies to demonstrate that the concentration of Lm does not exceed 100 CFU/g at the end of the shelf-life, taking into account foreseeable conditions of distributions, storage and use, including the use by consumers. However, this last part of the cold chain for food products is the most difficult to capture and control. For this purpose, the European Union Reference Laboratory for Lm (EURL Lm) launched an inquiry to its National Reference Laboratory network and reviewed the scientific literature from 2002 to 2020. The outcomes were integrated in the technical guidance document of the EURL Lm to assess shelf-life of RTEF which resulted in the recommendation to use 10 °C as the reference temperature to simulate the reasonably foreseen storage conditions in domestic refrigerators.
摘要：
单核细胞增生李斯特菌（Lm）是一种能够在冷藏温度下生长的致病菌，在环境中广泛分布。这种细菌容易污染各种食品，其中冷藏即食食品（RTEF）可能对公众健康构成风险。在欧洲,食品经营者（FBO）应确保食品符合法规（EC）N°2073/2005中规定的相关微生物标准。Lm的食品安全标准在RTEF中定义了整个保质期。FBOs应实施研究，以证明Lm的浓度在保质期结束时不超过100CFU/g，考虑到可预见的分配条件，储存和使用，包括消费者的使用。然而,食品冷链的最后一部分是最难捕获和控制的。为此,欧盟Lm参考实验室（EURLLm）对其国家参考实验室网络进行了调查，并审查了2002年至2020年的科学文献。结果被整合到EURLLm的技术指导文件中，以评估RTEF的保质期，从而建议使用10°C作为参考温度，以模拟家用冰箱中合理预见的储存条件。
