Abstract：
Diabetic cardiomyopathy (DCM) is one of the serious complications of type 2 diabetes mellitus. Vasant Kusumakar Rasa (VKR) is a Herbo-metallic formulation reported in Ayurveda, an Indian system of medicine. The present work was designed to study the effect of VKR in cardiomyopathy in type 2 diabetic rats. Diabetes was induced by feeding a high-fat diet (HFD) for 2 weeks followed by streptozotocin (STZ) administration (35 mg/kg i.p.). VKR was administered orally at dose of 28 and 56 mg/kg once a day for 16 weeks. The results of the study indicated that VKR treatment significantly improved the glycemic and lipid profile, serum insulin, CK-MB, LDH, and cardiac troponin-I when compared to diabetic control animals. VKR treatment in rats significantly improved the hemodynamic parameters and cardiac tissue levels of TNF-α, IL-1β, and IL- 6 were also reduced. Antioxidant enzymes such as GSH, SOD, and catalase were improved in all treatment groups. Heart sections stained with H & E and Masson\'s trichome showed decreased damage to histoarchitecture of the myocardium. Expression of PI3K, Akt, and GLUT4 in the myocardium was upregulated after 16 weeks of VKR treatment. The study data suggested the cardioprotective capability of VKR in the management of diabetic cardiomyopathy in rats.
摘要：
糖尿病心肌病(DCM)是2型糖尿病的严重并发症之一。VasantKusumakarRasa（VKR）是阿育吠陀报道的一种Herbo金属配方，印度的医学系统。本研究旨在研究VKR在2型糖尿病大鼠心肌病中的作用。通过喂养高脂肪饮食（HFD）2周，然后施用链脲佐菌素（STZ）（35mg/kg腹膜内）来诱导糖尿病。VKR以28和56mg/kg的剂量每天一次口服给药16周。研究结果表明，VKR治疗显着改善了血糖和血脂状况，血清胰岛素，CK-MB,LDH,和与糖尿病对照动物相比的心肌肌钙蛋白-I。VKR治疗显著改善大鼠血流动力学参数和心肌组织TNF-α水平,IL-1β,IL-6也减少了。抗氧化酶如GSH，SOD,所有治疗组过氧化氢酶均得到改善。用H＆E和Masson的毛状体染色的心脏切片显示对心肌组织结构的损害减少。PI3K的表达,Akt,VKR治疗16周后，心肌中的GLUT4上调。研究数据表明VKR在治疗大鼠糖尿病性心肌病中具有心脏保护能力。
