Abstract:
Acute lymphoblastic leukemia (ALL) is the most common type of leukemia in childhood and rare in adults, while acute myeloid leukemia (AML) is less common in children and more common in older adults. The aim of the study was to present our experience for the diagnostic of leukemia by using the classic and molecular cytogenetic methods. The study was conducted between 2009 and 2019 within the Classic and Molecular Genetic Laboratory of the Oncohematology Department from the Louis Ţurcanu Emergency Hospital for Children, Timişoara, Romania. The study group included 337 children and adults, evaluated between 2009 and 2019. By using the conventional and molecular cytogenetic technique, the cytogenetic anomalies found were 35 numerical chromosomal abnormalities, 10 (9;22)(q34;q11) [four ALL, one AML, five chronic myeloid leukemia (CML)] translocations, nine (15;17)(q24;q21) translocations, three (14;14)(q11;q32) translocations, two (4;11)(q21;q23) translocations, one (1;14)(p32;q11) translocation, one (7;14)(qter;q11) translocation, one (8;21)(q22;q22) translocation, one (9;14)(p12;q32) translocation, seven rearrangements of the MLL gene and two rearrangements of the core-binding factor subunit beta∕myosin heavy chain 11 (CBFB∕MYH11) gene. The use of conventional and molecular cytogenetic analysis is one of the most important prognostic indicators in acute leukemia patients, allowing the identification of biologically distinct subtypes of disease and selection of appropriate treatment approaches.
摘要:
急性淋巴细胞白血病(ALL)是儿童和成人罕见的最常见的白血病类型,而急性髓细胞性白血病(AML)在儿童中不太常见,在老年人中更常见。该研究的目的是通过使用经典和分子细胞遗传学方法来介绍我们诊断白血病的经验。这项研究是在2009年至2019年之间在路易斯·乌尔卡努儿童医院的肿瘤学系经典和分子遗传实验室进行的,提米索拉,罗马尼亚。研究组包括337名儿童和成人,2009年至2019年评估。通过使用常规和分子细胞遗传学技术,发现的细胞遗传学异常是35个数字染色体异常,10(9;22)(q34;q11)[四个,一个AML,五次慢性粒细胞白血病(CML)]易位,九(15;17)(q24;q21)易位,三(14;14)(q11;q32)易位,两个(4;11)(q21;q23)易位,一(1;14)(p32;q11)易位,一(7;14)(qter;q11)易位,一个(8;21)(q22;q22)易位,一(9;14)(p12;q32)易位,MLL基因的七个重排和核心结合因子亚基β/肌球蛋白重链11(CBFB/MYH11)基因的两个重排。使用常规和分子细胞遗传学分析是急性白血病患者最重要的预后指标之一,允许识别生物学上不同的疾病亚型并选择适当的治疗方法。