Abstract：
Here, we establish that plasticity exists within the postnatal enteric nervous system by demonstrating the reinnervation potential of post-mitotic enteric neurons (ENs). Employing BAF53b-Cre mice for selective neuronal tracing, the reinnervation capabilities of mature postnatal ENs are shown across multiple model systems. Isolated ENs regenerate neurites in vitro, with neurite complexity and direction influenced by contact with enteric glial cells (EGCs). Nerve fibers from transplanted ENs exclusively interface and travel along EGCs within the muscularis propria. Resident EGCs persist after Cre-dependent ablation of ENs and govern the architecture of the myenteric plexus for reinnervating ENs, as shown by nerve fiber projection tracing. Transplantation and optogenetic experiments in vivo highlight the rapid reinnervation potential of post-mitotic neurons, leading to restored gut muscle contractile activity within 2 weeks. These studies illustrate the structural and functional reinnervation capacity of post-mitotic ENs and the critical role of EGCs in guiding and patterning their trajectories.
摘要：
这里,通过证明有丝分裂后肠神经元（EN）的神经支配潜力，我们确定可塑性存在于出生后肠神经系统中。使用BAF53b-Cre小鼠进行选择性神经元示踪，在多个模型系统中显示了成熟的产后EN的神经支配能力。分离的ENs在体外再生神经突，神经突复杂性和方向受与肠胶质细胞（EGC）接触的影响。来自移植EN的神经纤维仅与固有肌层内的EGC连接并沿着EGC传播。居民EGCs在Cre依赖性ENs消融后持续存在，并控制肠肌间神经丛的结构以恢复ENs的神经，如神经纤维投影追踪所示。体内移植和光遗传学实验突出了有丝分裂后神经元的快速神经支配潜力，导致2周内恢复肠道肌肉收缩活动。这些研究说明了有丝分裂后ENs的结构和功能神经支配能力以及EGC在引导和图案化其轨迹中的关键作用。
