PDF(Pubmed)
Abstract：
UNASSIGNED: Despite mounting evidence of gut-brain involvement in psychiatric conditions, functional data remain limited, and analyses of other microbial niches, such as the vaginal microbiota, are lacking in relation to mental health. This aim of this study was to investigate if the connections between the gut microbiome and mental health observed in populations with a clinical diagnosis of mental illness extend to healthy women experiencing stress and depressive symptoms. Additionally, this study examined the functional pathways of the gut microbiota according to the levels of psychological symptoms. Furthermore, the study aimed to explore potential correlations between the vaginal microbiome and mental health parameters in young women without psychiatric diagnoses.
UNASSIGNED: In this cross-sectional study, 160 healthy Danish women (aged 18-40 years) filled out questionnaires with validated scales measuring symptoms of stress and depression and frequency of dietary intake. Fecal and vaginal microbiota samples were collected at the beginning of the menstrual cycle and vaginal samples were also collected at cycle day 8-12 and 18-22. Shotgun metagenomic profiling of the gut and vaginal microbiome was performed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for functional profiling and 56 Gut Brain Modules were analyzed in the fecal samples.
UNASSIGNED: The relative abundance in the gut of the genera Escherichia, Parabacteroides, and Shigella was higher in women with elevated depressive symptoms. Women with high perceived stress showed a tendency of increased abundance of Escherichia, Shigella, and Blautia. Amongst others, the potentially pathogenic genera, Escherichia and Shigella correlate with alterations in the neuroactive pathways such as the glutamatergic, GABAeric, dopaminergic, and Kynurenine pathways. Vaginosis symptoms were more prevalent in women reporting high levels of stress and depressive symptoms.
UNASSIGNED: The findings of this study support the concept of a microbiota-associated effect on the neuroactive pathways even in healthy young women. This suggest, that targeting the gut microbiome could be a promising approach for future psychiatric interventions.
UNASSIGNED: In this cross-sectional study, 160 healthy Danish women (aged 18-40 years) filled out questionnaires with validated scales measuring symptoms of stress and depression and frequency of dietary intake. Fecal and vaginal microbiota samples were collected at the beginning of the menstrual cycle and vaginal samples were also collected at cycle day 8-12 and 18-22. Shotgun metagenomic profiling of the gut and vaginal microbiome was performed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for functional profiling and 56 Gut Brain Modules were analyzed in the fecal samples.
UNASSIGNED: The relative abundance in the gut of the genera Escherichia, Parabacteroides, and Shigella was higher in women with elevated depressive symptoms. Women with high perceived stress showed a tendency of increased abundance of Escherichia, Shigella, and Blautia. Amongst others, the potentially pathogenic genera, Escherichia and Shigella correlate with alterations in the neuroactive pathways such as the glutamatergic, GABAeric, dopaminergic, and Kynurenine pathways. Vaginosis symptoms were more prevalent in women reporting high levels of stress and depressive symptoms.
UNASSIGNED: The findings of this study support the concept of a microbiota-associated effect on the neuroactive pathways even in healthy young women. This suggest, that targeting the gut microbiome could be a promising approach for future psychiatric interventions.
摘要：
■尽管有越来越多的证据表明肠道-大脑参与了精神疾病，功能数据仍然有限，以及其他微生物生态位的分析，例如阴道微生物群，缺乏与心理健康的关系。这项研究的目的是调查在患有精神疾病的临床诊断人群中观察到的肠道微生物组和心理健康之间的联系是否扩展到经历压力和抑郁症状的健康女性。此外,这项研究根据心理症状的水平检查了肠道微生物群的功能途径。此外，该研究旨在探讨未进行精神病诊断的年轻女性阴道微生物组和心理健康参数之间的潜在相关性.
■在这项横断面研究中，160名健康的丹麦女性（年龄在18-40岁之间）填写了经过验证的量表问卷，以测量压力和抑郁的症状以及饮食摄入的频率。在月经周期开始时收集粪便和阴道微生物群样品,并且还在周期第8-12天和第18-22天收集阴道样品。进行肠道和阴道微生物组的鸟枪宏基因组分析。使用京都基因和基因组百科全书(KEGG)进行功能分析,并在粪便样品中分析56个肠道脑模块。
■大肠杆菌属肠道中的相对丰度，副杆菌属,在抑郁症状升高的女性中，志贺氏菌较高。具有高感知压力的女性表现出大肠杆菌丰度增加的趋势，志贺氏菌,还有Blautia.其中,潜在致病属，埃希氏菌和志贺氏菌与神经活性通路的改变相关，如谷氨酸能，GABAeric,多巴胺能,和犬尿氨酸途径。阴道病症状在报告高水平压力和抑郁症状的女性中更为普遍。
■这项研究的结果支持了即使在健康的年轻女性中，微生物群对神经活性通路的影响的概念。这表明,针对肠道微生物组可能是未来精神病学干预的一种有希望的方法。
■在这项横断面研究中，160名健康的丹麦女性（年龄在18-40岁之间）填写了经过验证的量表问卷，以测量压力和抑郁的症状以及饮食摄入的频率。在月经周期开始时收集粪便和阴道微生物群样品,并且还在周期第8-12天和第18-22天收集阴道样品。进行肠道和阴道微生物组的鸟枪宏基因组分析。使用京都基因和基因组百科全书(KEGG)进行功能分析,并在粪便样品中分析56个肠道脑模块。
■大肠杆菌属肠道中的相对丰度，副杆菌属,在抑郁症状升高的女性中，志贺氏菌较高。具有高感知压力的女性表现出大肠杆菌丰度增加的趋势，志贺氏菌,还有Blautia.其中,潜在致病属，埃希氏菌和志贺氏菌与神经活性通路的改变相关，如谷氨酸能，GABAeric,多巴胺能,和犬尿氨酸途径。阴道病症状在报告高水平压力和抑郁症状的女性中更为普遍。
■这项研究的结果支持了即使在健康的年轻女性中，微生物群对神经活性通路的影响的概念。这表明,针对肠道微生物组可能是未来精神病学干预的一种有希望的方法。
