PDF(Pubmed)
Abstract：
The benefits of physical exercise on human health make it desirable to identify new approaches that would mimic or potentiate the effects of exercise to treat metabolic diseases. However, whether far-infrared (FIR) hyperthermia therapy could be used as exercise mimetic to realize wide-ranging metabolic regulation, and its underling mechanisms remain unclear. Here, a specific far-infrared (FIR) rays generated from graphene-based hyperthermia devices might promote exercise capacity and metabolisms. The material characterization showed that the graphene synthesized by chemical vapour deposition (CVD) was different from carbon fiber, with single-layer structure and high electrothermal transform efficiency. The emission spectra generated by graphene-FIR device would maximize matching those adsorbed by tissues. Graphene-FIR enhanced both core and epidermal temperatures, leading to increased blood flow in the femoral muscle and the abdominal region. The combination of microbiomic and metabolomic analysis revealed that graphene-FIR modulates the metabolism of the gut-muscle axis. This modulation was characterized by an increased abundance of short-chain fatty acids (SCFA)-producing bacteria and AMP, while lactic acid levels decreased. Furthermore, the principal routes involved in glucose metabolism, such as glycolysis and gluconeogenesis, were found to be altered. Graphene-FIR managed to stimulate AMPK activity by activating GPR43, thus enhancing muscle glucose uptake. Furthermore, a microbiota disorder model also demonstrated that the graphene-FIR effectively restore the exercise endurance with enhanced p-AMPK and GLUT4. Our results provided convincing evidence that graphene-based FIR therapy promoted exercise capacity and glucose metabolism via AMPK in gut-muscle axis. These novel findings regarding the therapeutic effects of graphene-FIR suggested its potential utility as a mimetic agent in clinical management of metabolic disorders.
摘要：
体育锻炼对人类健康的益处使得需要确定能够模拟或增强锻炼效果以治疗代谢疾病的新方法。然而,远红外（FIR）热疗是否可以用作运动模拟来实现广泛的代谢调节，其基本机制仍不清楚。这里,基于石墨烯的热疗设备产生的特定远红外（FIR）射线可能会促进运动能力和新陈代谢。材料表征表明,化学气相沉积法(CVD)合成的石墨烯不同于碳纤维,单层结构，电热变换效率高。由石墨烯-FIR装置产生的发射光谱将最大化匹配被组织吸附的那些。石墨烯-FIR提高了核心和表皮温度，导致股肌肉和腹部区域的血流量增加。微生物组学和代谢组学分析的结合表明石墨烯-FIR调节肠-肌肉轴的代谢。这种调节的特征是产生短链脂肪酸（SCFA）的细菌和AMP的丰度增加，而乳酸水平下降。此外，涉及葡萄糖代谢的主要途径，比如糖酵解和糖异生，被发现被改变了。石墨烯-FIR通过激活GPR43设法刺激AMPK活性,从而增强肌肉葡萄糖摄取。此外，微生物群紊乱模型还表明，石墨烯-FIR通过增强p-AMPK和GLUT4有效恢复运动耐力。我们的结果提供了令人信服的证据，表明基于石墨烯的FIR疗法通过肠-肌轴中的AMPK促进运动能力和葡萄糖代谢。这些关于石墨烯-FIR的治疗效果的新发现表明其作为代谢紊乱的临床管理中的模拟剂的潜在效用。
