目的:腹膜癌病(PC)在治疗晚期,实体瘤,传统疗法受药物渗透性差的限制。我们使用人腹腔模型评估了一种新型的高温加压腹膜内气溶胶化疗(HPIPAC)系统对AGS胃癌细胞的疗效。
方法:使用模拟人腹腔和AGS胃癌细胞系培养皿的模型来评估HPIPAC系统的功效。测量细胞活力以评估HPIPAC在6种不同条件下的影响:仅加热,PIPAC与紫杉醇(PTX),仅PTX,单独使用生理盐水(NS),用NS加热,以及带有PTX的HPIPAC。
结果:结果显示HPIPAC与PTX联用时细胞活力显著降低,表明增强的细胞毒性作用。治疗后立即,平均细胞活力为66.6%,48小时后下降到49.2%,120小时后下降到19.6%,证明了治疗的持续疗效。相比之下,对照组显示细胞活力恢复;仅加热显示细胞活力从90.8%增加到94.4%,PIPAC与PTX从82.7%到89.7%,PTX仅从73.3%到74.8%,NS仅从90.9%到98.3%,和加热NS从74.4%到84.7%。
结论:使用PTX的HPIPAC系统在治疗胃癌PC中显示出一种有希望的方法,显著降低细胞活力。尽管有一定的局限性,这项研究强调了该系统提高治疗效果的潜力。未来的努力应集中在完善HPIPAC并验证其在临床环境中的有效性。
OBJECTIVE: Peritoneal carcinomatosis (PC) presents a major challenge in the treatment of late-stage, solid tumors, with traditional therapies limited by poor drug penetration. We evaluated a novel hyperthermic pressurized intraperitoneal aerosol chemotherapy (HPIPAC) system using a human abdominal cavity model for its efficacy against AGS gastric cancer cells.
METHODS: A model simulating the human abdominal cavity and AGS gastric cancer cell line cultured dishes were used to assess the efficacy of the HPIPAC system. Cell viability was measured to evaluate the impact of HPIPAC under 6 different conditions: heat alone, PIPAC with paclitaxel (PTX), PTX alone, normal saline (NS) alone, heat with NS, and HPIPAC with PTX.
RESULTS: Results showed a significant reduction in cell viability with HPIPAC combined with PTX, indicating enhanced cytotoxic effects. Immediately after treatment, the average cell viability was 66.6%, which decreased to 49.2% after 48 hours and to a further 19.6% after 120 hours of incubation, demonstrating the sustained efficacy of the treatment. In contrast, control groups exhibited a recovery in cell viability; heat alone showed cell viability increasing from 90.8% to 94.4%, PIPAC with PTX from 82.7% to 89.7%, PTX only from 73.3% to 74.8%, NS only from 90.9% to 98.3%, and heat with NS from 74.4% to 84.7%.
CONCLUSIONS: The HPIPAC system with PTX exhibits a promising approach in the treatment of PC in gastric cancer, significantly reducing cell viability. Despite certain limitations, this study highlights the system\'s potential to enhance treatment outcomes. Future efforts should focus on refining HPIPAC and validating its effectiveness in clinical settings.