Abstract：
Getah virus (GETV) is an emerging mosquito-borne virus with economic impact on the livestock industry in East Asia. In this study, we successfully produced GETV virus-like particles (VLPs) in insect cells using the baculovirus expression vector system. We show that the GETV envelope glycoproteins were successfully expressed at the surface of the insect cell and were glycosylated. VLPs were isolated from the culture fluid as enveloped particles of 60-80 nm in diameter. Two 1 µg vaccinations with this GETV VLP vaccine, without adjuvant, generated neutralizing antibody responses and protected wild-type C57/BL6 mice against GETV viremia and arthritic disease. The GETV VLP vaccine may find application as a horse and/or pig vaccine in the future.
摘要：
Getah病毒（GETV）是一种新兴的蚊媒病毒，对东亚的畜牧业产生经济影响。在这项研究中,我们使用杆状病毒表达载体系统成功地在昆虫细胞中产生了GETV病毒样颗粒（VLP）。我们证明GETV包膜糖蛋白在昆虫细胞表面成功表达并被糖基化。从培养液中分离出直径为60-80nm的包封颗粒的VLP。用这种GETVVLP疫苗接种了两次1µg疫苗，没有佐剂，产生中和抗体反应，并保护野生型C57/BL6小鼠抵抗GETV病毒血症和关节炎疾病。GETVLP疫苗可在未来用作马和/或猪疫苗。
