PDF(Pubmed)
Abstract：
Populations in isolated and small fragments lose genetic variability very fast and are usually of conservation concern because they are at greater risk of local extinction. The largest native deer in South America, Blastocerus dichotomus (Illiger, 1815), is a Vulnerable species according to the IUCN categorization, which inhabits tropical and subtropical swampy areas. In Argentina, its presence has been restricted to four isolated fragments. Here we examine the genetic diversity and differentiation among three of them, including the three different patches that form the southernmost population, using 18 microsatellite markers genotyped by Amplicon Sequencing of DNA extracted from fecal samples. Genetic diversity was low (HE < 0.45) in all three populations studied. We found three genetic clusters compatible with the geographic location of the samples. We also found a metapopulation dynamics that involves the patches that make up the southernmost population, with evidence of a barrier to gene flow between two of them. Our results point to the creation of a corridor as a necessary and urgent management action. This is the first study, at the population level, employing microsatellite genotyping by Amplicon Sequencing with non-invasive samples in an endangered species.
摘要：
孤立和小片段中的种群很快失去遗传变异性，通常受到保护关注，因为它们面临更大的局部灭绝风险。南美最大的本地鹿，双歧杆菌（Illiger，1815),根据世界自然保护联盟的分类，是一个脆弱的物种，栖息在热带和亚热带沼泽地区。在阿根廷,它的存在仅限于四个孤立的片段。在这里，我们研究了其中三个之间的遗传多样性和分化，包括构成最南端人口的三个不同的斑块，使用通过扩增子测序从粪便样品中提取的DNA进行基因分型的18个微卫星标记。在所研究的所有三个种群中，遗传多样性都很低（HE<0.45）。我们发现了三个与样本地理位置相符的遗传簇。我们还发现了一个种群动态，它涉及构成最南端人口的斑块，有证据表明它们之间的基因流动存在障碍。我们的结果表明，建立走廊是必要和紧急的管理行动。这是第一项研究，在人口层面，通过扩增子测序对濒危物种的非侵入性样品进行微卫星基因分型。
