Abstract：
The land application of livestock manure has been widely acknowledged as a beneficial approach for nutrient recycling and environmental protection. However, the impact of residual antibiotics, a common contaminant of manure, on the degradation of organic compounds and nutrient release in Eutric Regosol is not well understood. Here, we studied, how oxytetracycline (OTC) and ciprofloxacin (CIP) affect the decomposition, microbial community structure, extracellular enzyme activities and nutrient release from cattle and pig manure using litterbag incubation experiments. Results showed that OTC and CIP greatly inhibited livestock manure decomposition, causing a decreased rate of carbon (28%-87%), nitrogen (15%-44%) and phosphorus (26%-43%) release. The relative abundance of gram-negative (G-) bacteria was reduced by 4.0%-13% while fungi increased by 7.0%-71% during a 28-day incubation period. Co-occurrence network analysis showed that antibiotic exposure disrupted microbial interactions, particularly among G- bacteria, G+ bacteria, and actinomycetes. These changes in microbial community structure and function resulted in decreased activity of urease, β-1,4-N-acetyl-glucosaminidase, alkaline protease, chitinase, and catalase, causing reduced decomposition and nutrient release in cattle and pig manures. These findings advance our understanding of decomposition and nutrient recycling from manure-contaminated antibiotics, which will help facilitate sustainable agricultural production and soil carbon sequestration.
摘要：
畜禽粪便的土地施用已被广泛认为是养分回收和环境保护的有益方法。然而,残留抗生素的影响，一种常见的粪便污染物，对EutricRegosol中有机化合物的降解和养分释放的了解尚不清楚。这里,我们研究过,土霉素（OTC）和环丙沙星（CIP）如何影响分解，微生物群落结构,使用垃圾袋孵化实验，牛和猪粪中的胞外酶活性和养分释放。结果表明，OTC和CIP显著抑制畜禽粪便分解，导致碳含量下降（28%-87%），氮(15%-44%)和磷(26%-43%)释放。在28天的孵育期内，革兰氏阴性（G-）细菌的相对丰度降低了4.0％-13％，而真菌的相对丰度增加了7.0％-71％。共现网络分析显示，抗生素暴露破坏了微生物相互作用，特别是在G-细菌中，G+细菌，和放线菌.微生物群落结构和功能的这些变化导致脲酶活性降低，β-1,4-N-乙酰氨基葡萄糖苷酶,碱性蛋白酶，几丁质酶，和过氧化氢酶,导致牛和猪粪中分解和营养释放减少。这些发现促进了我们对粪肥污染抗生素的分解和营养循环的理解，这将有助于促进可持续的农业生产和土壤碳固存。
