关键词: Capsid Condensation Fluorescence recovery after photobleaching HIV-1 In-cell condensation Intrinsic disorder Liquid–liquid phase separation Nucleocapsid Virus assembly

Mesh : Humans HIV Infections / virology HIV-1 / genetics physiology Phase Separation RNA, Viral / genetics Virus Replication

来  源:   DOI:10.1016/j.ymeth.2024.07.002

Abstract:
This article reviews tried-and-tested methodologies that have been employed in the first studies on phase separating properties of structural, RNA-binding and catalytic proteins of HIV-1. These are described here to stimulate interest for any who may want to initiate similar studies on virus-mediated liquid-liquid phase separation. Such studies serve to better understand the life cycle and pathogenesis of viruses and open the door to new therapeutics.
摘要:
本文回顾了久经考验的方法,这些方法已在结构相分离特性的最初研究中使用,HIV-1的RNA结合和催化蛋白。这里描述这些是为了激发任何可能想要对病毒介导的液-液相分离进行类似研究的人的兴趣。这些研究有助于更好地了解病毒的生命周期和发病机理,并为新疗法打开大门。
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