{Reference Type}: Journal Article
{Title}: Methodological approaches to studying phase separation and HIV-1 replication: Current and future perspectives.
{Author}: Mouland AJ;Chau BA;Uversky VN;
{Journal}: Methods
{Volume}: 229
{Issue}: 0
{Year}: 2024 Sep 15
{Factor}: 4.647
{DOI}: 10.1016/j.ymeth.2024.07.002
{Abstract}: This article reviews tried-and-tested methodologies that have been employed in the first studies on phase separating properties of structural, RNA-binding and catalytic proteins of HIV-1. These are described here to stimulate interest for any who may want to initiate similar studies on virus-mediated liquid-liquid phase separation. Such studies serve to better understand the life cycle and pathogenesis of viruses and open the door to new therapeutics.