PDF(Pubmed)
Abstract：
Egg quality in fishes is commonly determined by fertilisation success and cleavage patterns as a phenotypic outcome of underlying regulatory mechanisms. Although these phenotypic estimators of egg quality are useful in farming conditions, these \"good quality\" egg batches do not always translate to good larval growth and survival. The identification of genes involved in embryonic development may help find links between genetic factors of maternal origin and egg quality. Herein, the relative expression of seven stage-specific developmental genes of Atlantic cod was analysed using quantitative PCR to understand the function during embryogenesis and its relationship with egg quality. Genes ccnb2 and pvalb1 showed significant differential expression between developmental stages and significant upregulation from blastula and somite stages, respectively. The comparison of spawning batches showed that the relative gene expression of genes ccnb2, acta, tnnt3 and pvalb1 was significantly higher from the middle of the spawning season where phenotypic quality estimators establish the best egg quality. Moreover, a positive significant correlation was observed between quality estimators based on egg morphology and the genetic expression of genes acta and acta1 during somitogenesis. This study suggests that the combination of quality estimators, genetics and batch timing could help optimise reproductive protocols for commercial stocks of Atlantic cod.
摘要：
鱼类的卵质量通常取决于受精成功和卵裂模式，这是潜在调节机制的表型结果。尽管这些鸡蛋质量的表型估计器在农业条件下很有用，这些“优质”的卵批次并不总是转化为良好的幼虫生长和存活。鉴定参与胚胎发育的基因可能有助于发现母源遗传因素与卵子质量之间的联系。在这里，使用定量PCR分析了大西洋鳕鱼的七个阶段特异性发育基因的相对表达，以了解胚胎发生过程中的功能及其与卵质量的关系。基因ccnb2和pvalb1在发育阶段之间显示出显着差异表达，并且从囊胚和体细胞阶段显着上调，分别。产卵批次的比较表明，基因ccnb2，acta，tnnt3和pvalb1从产卵季节的中期开始显着升高，表型质量估算器确定了最佳的卵质量。此外，在产卵过程中，基于卵形态的质量估计量与基因acta和acta1的遗传表达之间呈正相关。这项研究表明，质量估计器的组合，遗传学和批次时间安排可以帮助优化大西洋鳕鱼商业种群的繁殖方案。
