PDF(Pubmed)
Abstract：
Deoxynivalenol (DON) is a mycotoxin produced by Fusarium graminearum, and curcumin (CUR) is a natural polyphenolic compound found in turmeric. However, the combined treatment of CUR and DON to explore the mitigating effect of CUR on DON and their combined mechanism of action is not clear. Therefore, in this study, we established four treatment groups (CON, CUR, DON and CUR + DON) to investigate their mechanism in the porcine intestinal epithelial cells (IPEC-J2). In addition, the cross-talk and alleviating potential of CUR interfering with DON-induced cytotoxic factors were evaluated by in vitro experiments; the results showed that CUR could effectively inhibit DON-exposed activated TNF-α/NF-κB pathway, attenuate DON-induced apoptosis, and alleviate DON-induced endoplasmic reticulum stress and oxidative stress through PERK/CHOP pathways, which were verified at both mRNA and protein levels. In conclusion, these promising findings may contribute to the future use of CUR as a novel feed additive to protect livestock from the harmful effects of DON.
摘要：
脱氧雪腐镰刀菌烯醇（DON）是由镰刀菌产生的一种霉菌毒素，姜黄素（CUR）是姜黄中发现的天然多酚化合物。然而,CUR与DON联合治疗探讨CUR对DON的缓解作用及其联合作用机制尚不明确。因此,在这项研究中，我们建立了四个治疗组(CON，CUR,DON和CUR+DON)研究其在猪肠上皮细胞(IPEC-J2)中的作用机制。此外,通过体外实验评估了CUR干扰DON诱导的细胞毒性因子的串扰和缓解潜力。结果表明，CUR可以有效抑制DON暴露的激活的TNF-α/NF-κB通路，减弱DON诱导的细胞凋亡，并通过PERK/CHOP通路缓解DON诱导的内质网应激和氧化应激,在mRNA和蛋白质水平上都得到了验证。总之,这些有希望的发现可能有助于将来将CUR用作新型饲料添加剂，以保护牲畜免受DON的有害影响。
