PDF(Pubmed)
Abstract：
Helicobacter pylori infection affects over 50% of the world\'s population and leads to chronic inflammation and gastric disorders, being the main pathogen correlated to gastric cancer development. Increasing antibiotic resistance levels are a major global concern and alternative treatments are needed. Soybean peptides and other compounds might be an alternative in the treatment to avoid, eradicate and/or control symptoms of H. pylori infection. This study aimed to characterize a lunasin-enriched soybean extract (LSE) using proteomics tools and to evaluate its antioxidant, anti-inflammatory and antibacterial properties against H. pylori infection. By LC-MS/MS analysis, 124 proteins were identified, with 2S albumin (lunasin and large-chain subunits) being the fourth most abundant protein (8.9%). Lunasin consists of 44 amino acid residues and an intramolecular disulfide bond. LSE at a low dose (0.0625 mg/mL) reduced ROS production in both H. pylori-infected and non-infected AGS gastric cells. This led to a significant reduction of 6.71% in the levels of pro-inflammatory interleukin (IL)-8. LSE also showed antibacterial activity against H. pylori, which can be attributed to other soybean proteins and phenolic compounds. Our findings suggest that LSE might be a promising alternative in the management of H. pylori infection and its associated symptoms.
摘要：
幽门螺杆菌感染影响了世界上50%以上的人口，并导致慢性炎症和胃部疾病，是与胃癌发展相关的主要病原体。增加抗生素抗性水平是全球关注的主要问题，需要替代疗法。大豆肽和其他化合物可能是治疗中避免的替代方法，根除和/或控制幽门螺杆菌感染的症状。本研究旨在使用蛋白质组学工具表征富含露纳辛的大豆提取物（LSE），并评估其抗氧化剂，抗幽门螺杆菌感染的抗炎和抗菌特性。通过LC-MS/MS分析,鉴定了124种蛋白质，其中2S白蛋白（lunasin和大链亚基）是第四丰富的蛋白质（8.9％）。Lunasin由44个氨基酸残基和一个分子内二硫键组成。低剂量（0.0625mg/mL）的LSE降低了幽门螺杆菌感染和未感染的AGS胃细胞中的ROS产生。这导致促炎性白介素(IL)-8水平显著降低6.71%。LSE还显示出对幽门螺杆菌的抗菌活性，这可以归因于其他大豆蛋白和酚类化合物。我们的研究结果表明，LSE可能是治疗幽门螺杆菌感染及其相关症状的一种有希望的替代方法。
