PDF(Pubmed)
Abstract：
The ongoing development of bacterial resistance to antibiotics is a global challenge. Research in that field is thus necessary. Analytical techniques are required for such a purpose. From this perspective, the focus was on atomic absorption spectrometry (AAS). Although it is old, AAS often offers unexpected potential. Of course, this should be exploited. The aim was therefore to demonstrate the versatility of the technique in antibacterial research. This is illustrated by various examples of its practical application. AAS can be used, for example, to confirm the identity of antibacterial compounds, for purity controls, or to quantify the antibiotics in pharmaceutical preparations. The latter allowed analysis without laborious sample preparation and without interference from other excipients. In addition, AAS can help elucidate the mode of action or resistance mechanisms. In this context, quantifying the accumulation of the antibiotic drug in the cell of (resistant) bacteria appears to play an important role. The general application of AAS is not limited to metal-containing drugs, but also enables the determination of some organic chemical antibiotics. Altogether, this perspective presents a range of applications for AAS in antibacterial research, intending to raise awareness of the method and may thus contribute to the fight against resistance.
摘要：
细菌对抗生素耐药性的持续发展是一个全球性挑战。因此，该领域的研究是必要的。为此目的需要分析技术。从这个角度来看,重点是原子吸收光谱法（AAS）。虽然它很旧,AAS通常提供意想不到的潜力。当然,这应该被利用。因此，目的是证明该技术在抗菌研究中的多功能性。这通过其实际应用的各种示例来说明。可以使用AAS，例如,为了确认抗菌化合物的身份，对于纯度控制，或量化药物制剂中的抗生素。后者允许分析而无需费力的样品制备并且不受其他赋形剂的干扰。此外,AAS可以帮助阐明作用模式或抵抗机制。在这种情况下，量化抗生素药物在（耐药）细菌细胞中的积累似乎起着重要作用。AAS的一般应用不仅限于含金属药物，还能测定一些有机化学抗生素。总之,这种观点提出了AAS在抗菌研究中的一系列应用，旨在提高对该方法的认识，从而可能有助于对抗抵抗。
