PDF(Pubmed)
Abstract：
As part of the multifaceted strategies developed to shape the common environmental policy, considerable attention is now being paid to assessing the degree of environmental degradation in soil under xenobiotic pressure. Bisphenol A (BPA) has only been marginally investigated in this ecosystem context. Therefore, research was carried out to determine the biochemical properties of soils contaminated with BPA at two levels of contamination: 500 mg and 1000 mg BPA kg-1 d.m. of soil. Reliable biochemical indicators of soil changes, whose activity was determined in the pot experiment conducted, were used: dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase, arylsulfatase, and β-glucosidase. Using the definition of soil health as the ability to promote plant growth, the influence of BPA on the growth and development of Zea mays, a plant used for energy production, was also tested. As well as the biomass of aerial parts and roots, the leaf greenness index (SPAD) of Zea mays was also assessed. A key aspect of the research was to identify those of the six remediating substances-molecular sieve, zeolite, sepiolite, starch, grass compost, and fermented bark-whose use could become common practice in both environmental protection and agriculture. Exposure to BPA revealed the highest sensitivity of dehydrogenases, urease, and acid phosphatase and the lowest sensitivity of alkaline phosphatase and catalase to this phenolic compound. The enzyme response generated a reduction in the biochemical fertility index (BA21) of 64% (500 mg BPA) and 70% (1000 mg BPA kg-1 d.m. of soil). The toxicity of BPA led to a drastic reduction in root biomass and consequently in the aerial parts of Zea mays. Compost and molecular sieve proved to be the most effective in mitigating the negative effect of the xenobiotic on the parameters discussed. The results obtained are the first research step in the search for further substances with bioremediation potential against both soil and plants under BPA pressure.
摘要：
作为制定共同环境政策的多方面战略的一部分，现在正在相当重视评估异生压力下土壤中环境退化的程度。在这种生态系统背景下，双酚A（BPA）仅得到了少量研究。因此,进行了研究，以确定在两种污染水平下被BPA污染的土壤的生化特性：500mg和1000mgBPAkg-1d.m.的土壤。土壤变化的可靠生化指标，在进行的盆栽实验中确定了其活性，使用：脱氢酶，过氧化氢酶,脲酶,酸性磷酸酶,碱性磷酸酶,芳基硫酸酯酶,和β-葡萄糖苷酶。将土壤健康定义为促进植物生长的能力，双酚A对玉米生长发育的影响，用于能源生产的工厂，也进行了测试。以及地上部分和根部的生物量，还评估了玉米的叶绿指数(SPAD)。该研究的一个关键方面是确定六种修复物质-分子筛，沸石，海泡石,淀粉，草堆肥,和发酵树皮——它们的使用可能在环境保护和农业中成为普遍做法。暴露于BPA显示脱氢酶的最高敏感性，脲酶,和酸性磷酸酶以及碱性磷酸酶和过氧化氢酶对该酚类化合物的最低敏感性。酶反应使生化肥力指数（BA21）降低了64％（500mgBPA）和70％（1000mgBPAkg-1d.m.的土壤）。BPA的毒性导致根生物量急剧减少，因此在玉米的地上部分也急剧减少。堆肥和分子筛被证明是最有效的减轻异种生物对所讨论参数的负面影响。获得的结果是寻找在BPA压力下对土壤和植物具有生物修复潜力的其他物质的第一步。
