PDF(Pubmed)
Abstract：
Skin aging is an inevitable and intricate process instigated, among others, by oxidative stress. The search for natural sources that inhibit this mechanism is a promising approach to preventing skin aging. The purpose of our study was to evaluate the composition of phenolic compounds in the micellar extract of Phaseolus vulgaris sprouts. The results of a liquid chromatography-mass spectrometry (LC-MS) analysis revealed the presence of thirty-two constituents, including phenolic acids, flavanols, flavan-3-ols, flavanones, isoflavones, and other compounds. Subsequently, the extract was assessed for its antioxidant, anti-inflammatory, anti-collagenase, anti-elastase, anti-tyrosinase, and cytotoxic properties, as well as for the evaluation of collagen synthesis. It was demonstrated that micellar extract from common bean sprouts has strong anti-aging properties. The performed WST-8 (a water-soluble tetrazolium salt) assay revealed that selected concentrations of extract significantly increased proliferation of human dermal fibroblasts compared to the control cells in a dose-dependent manner. A similar tendency was observed with respect to collagen synthesis. Our results suggest that micellar extract from Phaseolus vulgaris sprouts can be considered a promising anti-aging compound for applications in cosmetic formulations.
摘要：
皮肤老化是一个不可避免的复杂的过程，其中,通过氧化应激。寻找抑制这种机制的天然来源是防止皮肤老化的有希望的方法。我们研究的目的是评估菜豆的胶束提取物中酚类化合物的组成。液相色谱-质谱（LC-MS）分析的结果表明，存在32种成分，包括酚酸，黄烷醇,黄烷-3-醇，黄烷酮,异黄酮,和其他化合物。随后，对提取物的抗氧化剂进行了评估，抗炎,抗胶原酶,抗弹性蛋白酶,抗酪氨酸酶,和细胞毒性，以及胶原蛋白合成的评价。结果表明，普通豆芽的胶束提取物具有很强的抗衰老性能。进行的WST-8(水溶性四唑盐)测定显示,与对照细胞相比,选择浓度的提取物以剂量依赖性方式显著增加人真皮成纤维细胞的增殖。在胶原合成方面观察到类似的趋势。我们的结果表明，菜豆芽的胶束提取物可以被认为是一种有前途的抗衰老化合物，可用于化妆品配方中。
