PDF(Pubmed)
Abstract：
The Brassicaceae family, commonly referred to as cruciferous plants, is globally cultivated and consumed, with the Brassica genus being particularly renowned for its functional components. These vegetables are rich sources of nutrients and health-promoting phytochemicals, garnering increased attention in recent years. This study presents a comprehensive microscopic, chromatographic, and spectroscopic characterization of Brassica napus L. seeds from Kazakhstan aimed at elucidating their morphological features and chemical composition. Microscopic analysis revealed distinct localization of flavonoids, total lipids, and alkaloids. High-performance thin-layer chromatography (HPTLC) analysis of seed extracts demonstrated a complex chemical profile with significant quantities of non-polar compounds in the hexane extracts. Additionally, methanolic extracts revealed the presence of diverse chemical compounds, including alkaloids, flavonoids, and glucosinolates. The chemical composition exhibited varietal differences across different Brassica species, with B. napus L. seeds showing higher concentrations of bioactive compounds. Furthermore, liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QToF-MS) analysis provided insights into the chemical composition, with sinapine isomers, feruloyl, and sinapoyl choline derivatives as major compounds in the seeds. This study contributes to a better understanding of the chemical diversity and quality control methods\' approximations of B. napus L. seeds, highlighting their importance in functional food and nutraceutical applications.
摘要：
十字花科,通常被称为十字花科植物，在全球范围内种植和消费，芸苔属以其功能成分而闻名。这些蔬菜富含营养和促进健康的植物化学物质，近年来受到越来越多的关注。这项研究提出了一个全面的微观，色谱，哈萨克斯坦甘蓝型油菜种子的光谱表征旨在阐明其形态特征和化学成分。显微分析显示黄酮类化合物的不同定位，总脂质，和生物碱。种子提取物的高效薄层色谱（HPTLC）分析显示出复杂的化学特征，己烷提取物中存在大量非极性化合物。此外,甲醇提取物揭示了多种化合物的存在，包括生物碱,黄酮类化合物，和芥子油苷.化学成分在不同的芸苔属物种之间表现出品种差异，与B.napusL.种子显示更高浓度的生物活性化合物。此外，液相色谱-四极杆飞行时间质谱（LC-QToF-MS）分析提供了对化学成分的见解，与芥子碱异构体，阿魏酸,和芥子酰胆碱衍生物作为种子中的主要化合物。本研究有助于更好地理解欧洲油菜种子的化学多样性和质量控制方法，强调它们在功能性食品和营养品应用中的重要性。
