PDF(Pubmed)
Abstract：
The liver is the main organ responsible for the metabolism of ethanol, which suffers significantly as a result of tissue damage due to oxidative stress. It is known that C60 fullerenes are able to efficiently capture and inactivate reactive oxygen species in in vivo and in vitro systems. Therefore, the purpose of this study is to determine whether water-soluble C60 fullerene reduces the level of pathological process development in the liver of rats induced by chronic alcohol intoxication for 3, 6, and 9 months, depending on the daily dose (oral administration; 0.5, 1, and 2 mg/kg) of C60 fullerene throughout the experiment. In this context, the morphology of the C60 fullerene nanoparticles in aqueous solution was studied using atomic force microscopy. Such biochemical parameters of experimental animal blood as ALT (alanine aminotransferase), AST (aspartate aminotransferase), GGT (gamma-glutamyl transferase) and ALP (alkaline phosphatase) enzyme activities, CDT (carbohydrate-deficient transferrin) level, values of pro-antioxidant balance indicators (concentrations of H2O2 (hydrogen peroxide) and GSH (reduced glutathione), activities of CAT (catalase), SOD (superoxide dismutase) and GPx (selenium-dependent glutathione peroxidase)), and pathohistological and morphometric features of liver damage were analyzed. The most significant positive change in the studied biochemical parameters (up to 29 ± 2% relative to the control), as markers of liver damage, was recorded at the combined administration of alcohol (40% ethanol in drinking water) and water-soluble C60 fullerenes in the optimal dose of 1 mg/kg, which was confirmed by small histopathological changes in the liver of rats. The obtained results prove the prospective use of C60 fullerenes as powerful antioxidants for the mitigation of pathological conditions of the liver arising under prolonged alcohol intoxication.
摘要：
肝脏是负责乙醇代谢的主要器官，由于氧化应激导致的组织损伤而遭受严重损害。已知C60富勒烯能够有效地捕获和灭活体内和体外系统中的活性氧。因此,这项研究的目的是确定水溶性C60富勒烯是否降低慢性酒精中毒3、6和9个月大鼠肝脏病理过程发展的水平，取决于在整个实验中C60富勒烯的日剂量（口服给药；0.5、1和2mg/kg）。在这种情况下，用原子力显微镜研究了C60富勒烯纳米颗粒在水溶液中的形貌。实验动物血液的生化参数如ALT（丙氨酸氨基转移酶），AST（天冬氨酸转氨酶），GGT（γ-谷氨酰转移酶）和ALP（碱性磷酸酶）酶活性，CDT（碳水化合物缺乏转铁蛋白）水平，促抗氧化剂平衡指标的值（H2O2（过氧化氢）和GSH（还原型谷胱甘肽）的浓度，CAT（过氧化氢酶）的活性，SOD（超氧化物歧化酶）和GPx（硒依赖性谷胱甘肽过氧化物酶），并对肝损伤的病理组织学和形态学特征进行分析。研究的生化参数中最显著的积极变化（相对于对照高达29±2％），作为肝损伤的标志，在酒精（饮用水中40％乙醇）和水溶性C60富勒烯以1mg/kg的最佳剂量联合给药时记录，大鼠肝脏的小组织病理学改变证实了这一点。获得的结果证明了C60富勒烯作为强效抗氧化剂的预期用途，可缓解长期酒精中毒引起的肝脏病理状况。
