PDF(Pubmed)
Abstract：
In insects, biogenic amines function as neurotransmitters, neuromodulators, and neurohormones, influencing various behaviors, including those related to reproduction such as response to sex pheromones, oogenesis, oviposition, courtship, and mating. Octopamine (OA), an analog of the vertebrate norepinephrine, is synthesized from the biogenic amine tyramine by the enzyme tyramine β-hydroxylase (TβH). Here, we investigate the mechanisms and target genes underlying the role of OA in successful reproduction in females of Rhodnius prolixus, a vector of Chagas disease, by downregulating TβH mRNA expression (thereby reducing OA content) using RNA interference (RNAi), and in vivo and ex vivo application of OA. Injection of females with dsTβH impairs successful reproduction at least in part, by decreasing the transcript expression of enzymes involved in juvenile hormone biosynthesis, the primary hormone for oogenesis in R. prolixus, thereby interfering with oogenesis, ovulation and oviposition. This study offers valuable insights into the involvement of OA for successful reproduction in R. prolixus females. Understanding the reproductive biology of R. prolixus is crucial in a medical context for controlling the spread of the disease.
摘要：
在昆虫中，生物胺作为神经递质，神经调质，和神经激素，影响各种行为，包括与生殖有关的那些，例如对性信息素的反应，卵子发生，产卵,求爱,和交配。章鱼胺(OA),脊椎动物去甲肾上腺素的类似物，由生物胺酪胺通过酪胺β-羟化酶(TβH)合成。这里,我们调查的机制和靶基因的作用，OA在成功繁殖的女性的红藻，查加斯病的媒介,通过使用RNA干扰（RNAi）下调TβHmRNA表达（从而降低OA含量），以及OA的体内和离体应用。雌性注射dsTβH至少部分损害成功繁殖，通过减少参与幼体激素生物合成的酶的转录表达，主要的促生激素，从而干扰卵子发生，排卵和产卵。这项研究提供了有关OA参与雌性R.prolixus成功繁殖的宝贵见解。了解R.prolixus的生殖生物学在控制疾病传播的医学背景下至关重要。
